Shoot regeneration from cotyledonary leaf explants of jatropha curcas: A biodiesel plant

Nitish Chandramohana Kumar; K. G. Vijay Anand; Muppala P. Reddy

doi:10.1007/s11738-010-0479-9

Shoot regeneration from cotyledonary leaf explants of jatropha curcas: A biodiesel plant

Nitish Chandramohana Kumar, K. G. Vijay Anand, Muppala P. Reddy

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

A simple, high frequency, and reproducible method for plant regeneration through direct organogenesis from cotyledonary leaf explants of Jatropha curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) or 6-benzyl aminopurine (BAP). Medium containing TDZ has greater influence on regeneration as compared to BAP. The induced shoot buds were transferred to MS medium containing 10 lM kinetin (Kn), 4.5 lM BAP, and 5.5 lM a-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with different concentrations and combinations of BAP, indole-3-acetic acid (IAA), NAA, and indole-3-butyric acid (IBA). MS medium with 2.25 lM BAP and 8.5 lM IAA was found to be the best combination for shoot elongation. However, significant differences in plant regeneration and shoot elongation were observed among the genotypes studied. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing dif- ferent concentrations and combinations of IBA, IAA, and NAA for 4 days, followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg l-1 activated charcoal. Elongated shoot treated with 15 lM IBA, 5.7 lM IAA, and 11 lM NAA resulted in highest percent rooting. The rooted plants could be established in soil with more than 90% survival rate. The method developed may be useful in improvement of J. curcas through genetic modification. © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010.

Original language	English (US)
Pages (from-to)	917-924
Number of pages	8
Journal	Acta Physiologiae Plantarum
Volume	32
Issue number	5
DOIs	https://doi.org/10.1007/s11738-010-0479-9
State	Published - Mar 7 2010

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors gratefully acknowledge Council of Scientific and Industrial Research, New Delhi, India for financial assistance. Statistical analysis support from Mr. Keyur Shah and Mr. Amritpal singh, Assistant professor, Department of Agricultural Biotechnology, Anand Agricultural University, Anand, India is thankfully acknowledged.

ASJC Scopus subject areas

Plant Science
Agronomy and Crop Science
Physiology

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10.1007/s11738-010-0479-9

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title = "Shoot regeneration from cotyledonary leaf explants of jatropha curcas: A biodiesel plant",

abstract = "A simple, high frequency, and reproducible method for plant regeneration through direct organogenesis from cotyledonary leaf explants of Jatropha curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) or 6-benzyl aminopurine (BAP). Medium containing TDZ has greater influence on regeneration as compared to BAP. The induced shoot buds were transferred to MS medium containing 10 lM kinetin (Kn), 4.5 lM BAP, and 5.5 lM a-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with different concentrations and combinations of BAP, indole-3-acetic acid (IAA), NAA, and indole-3-butyric acid (IBA). MS medium with 2.25 lM BAP and 8.5 lM IAA was found to be the best combination for shoot elongation. However, significant differences in plant regeneration and shoot elongation were observed among the genotypes studied. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing dif- ferent concentrations and combinations of IBA, IAA, and NAA for 4 days, followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg l-1 activated charcoal. Elongated shoot treated with 15 lM IBA, 5.7 lM IAA, and 11 lM NAA resulted in highest percent rooting. The rooted plants could be established in soil with more than 90% survival rate. The method developed may be useful in improvement of J. curcas through genetic modification. {\textcopyright} Franciszek G{\'o}rski Institute of Plant Physiology, Polish Academy of Sciences, Krak{\'o}w 2010.",

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note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors gratefully acknowledge Council of Scientific and Industrial Research, New Delhi, India for financial assistance. Statistical analysis support from Mr. Keyur Shah and Mr. Amritpal singh, Assistant professor, Department of Agricultural Biotechnology, Anand Agricultural University, Anand, India is thankfully acknowledged.",

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Shoot regeneration from cotyledonary leaf explants of jatropha curcas: A biodiesel plant

Abstract

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