Sequenceserver: a modern graphical user interface for custom BLAST databases.

Anurag Priyam, Ben J Woodcroft, Vivek Rai, Ismail Moghul, Alekhya Mungala, Filip Ter, Hiten Chowdhary, Iwo Lukasz Pieniak, Mark Anthony Gibbins, HongKee Moon, Austin Davis-Richardson, Mahmut Uludag, Nathan S Watson-Haigh, Richard Challis, Hiroyuki Nakamura, Emeline Favreau, Esteban Gόmez Cifuentes, Tomás Pluskal, Guy Leonard, Wolfgang RumpfYannick Wurm

Research output: Contribution to journalArticlepeer-review

125 Scopus citations


Comparing newly obtained and previously known nucleotide and amino-acid sequences underpins modern biological research. BLAST is a well-established tool for such comparisons but is challenging to use on new datasets. We combined a user-centric design philosophy with sustainable software development approaches to create Sequenceserver, a tool for running BLAST and visually inspecting BLAST results for biological interpretation. Sequenceserver uses simple algorithms to prevent potential analysis errors, and provides flexible text-based and visual outputs to support researcher productivity. Our software can be rapidly installed for use by individuals or on shared servers. Sequenceserver is AGPLv3-licensed at
Original languageEnglish (US)
Pages (from-to)2922-2924
Number of pages3
JournalMolecular biology and evolution
Issue number12
StatePublished - Aug 14 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We thank the many Sequenceserver users and contributors for their input. During the creation of Sequenceserver YW was funded by an ERC grant to Laurent Keller. BJW was supported by the United States Department of Energy (DE-SC0004632). While writing this manuscript, YW and AP were supported by BBSRC (BB/K004204/1) and NERC (NE/L00626X/1).


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