Separation of proteases from yellowfin tuna spleen by ultrafiltration

Zhenyu Li, Wirote Youravong, Aran H-Kittikun*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Separation of protease, trypsin and chymotrypsin from yellowfin tuna spleen extract by ultrafiltration (UF) using regenerated cellulose membranes with molecular weight cut off (MWCO) 30 and 100 kDa was studied. The 100 kDa membrane had a higher transmission of enzymes than that of the 30 kDa membrane. The enzyme transmission varied from 0.01 to 0.18 and from 0.6 to 0.8 for the 30 kDa membrane and 100 kDa membrane, respectively. The protein transmission was about 0.8 for both membranes. Increasing cross-flow rate and transmembrane pressure (TMP) increased permeate flux. The limiting fluxes at cross-flow rate 120, 240 and 360 L/h for the 30 kDa membrane were 17.3, 43.9 and 54.7 L/m2 h, respectively and the limiting fluxes at the same flow rate for 100 kDa membrane were 34.1, 51.1 and 68.4 L/m2 h, respectively. The separation of these proteases was achieved using the 30 kDa membrane. The purities of proteases were increased more than ten times at TMP 1.5 bar and cross-flow rate 360 L/h by diafiltration using 30 kDa membrane.

Original languageEnglish (US)
Pages (from-to)2364-2370
Number of pages7
JournalBioresource technology
Issue number18
StatePublished - Dec 2006
Externally publishedYes


  • Chymotrypsin
  • Diafiltration
  • Proteases
  • Trypsin
  • Tuna spleen
  • Ultrafiltration

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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