Semipreparative capillary electrochromatography

J. R. Chen; R. N. Zare; E. C. Peters; F. Svec; J. J. Frechét

doi:10.1021/ac001302m

Semipreparative capillary electrochromatography

J. R. Chen, R. N. Zare^*, E. C. Peters, F. Svec, J. J. Frechét

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Capillaries with inner diameters of 550 μm have successfully been packed with 1.5-μm octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate and trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly-(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 μL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.

Original language	English (US)
Pages (from-to)	1987-1992
Number of pages	6
Journal	ANALYTICAL CHEMISTRY
Volume	73
Issue number	9
DOIs	https://doi.org/10.1021/ac001302m
State	Published - May 1 2001
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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title = "Semipreparative capillary electrochromatography",

abstract = "Capillaries with inner diameters of 550 μm have successfully been packed with 1.5-μm octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate and trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly-(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 μL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.",

author = "Chen, {J. R.} and Zare, {R. N.} and Peters, {E. C.} and F. Svec and Frech{\'e}t, {J. J.}",

year = "2001",

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day = "1",

doi = "10.1021/ac001302m",

language = "English (US)",

volume = "73",

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TY - JOUR

T1 - Semipreparative capillary electrochromatography

AU - Chen, J. R.

AU - Zare, R. N.

AU - Peters, E. C.

AU - Svec, F.

AU - Frechét, J. J.

PY - 2001/5/1

Y1 - 2001/5/1

N2 - Capillaries with inner diameters of 550 μm have successfully been packed with 1.5-μm octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate and trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly-(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 μL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.

AB - Capillaries with inner diameters of 550 μm have successfully been packed with 1.5-μm octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate and trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly-(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 μL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.

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U2 - 10.1021/ac001302m

DO - 10.1021/ac001302m

M3 - Article

C2 - 11354480

AN - SCOPUS:0035326448

SN - 0003-2700

VL - 73

SP - 1987

EP - 1992

JO - ANALYTICAL CHEMISTRY

JF - ANALYTICAL CHEMISTRY

IS - 9

ER -

Semipreparative capillary electrochromatography

Abstract

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