TY - GEN
T1 - Second harmonic phase microscopy of collagen fibers
AU - Shaffer, Etienne
AU - Marquet, Pierre
AU - Depeursinge, Christian
PY - 2011
Y1 - 2011
N2 - In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional information, inaccessible to incoherent imaging schemes. Last year, we have reported how the SHG phase makes possible real-time nanometric 3D-tracking of SHG emitters, such as nanoparticles (Shaffer et al., Opt. Express 18, p.17392-17403, 2010). Here, we investigate the phase of second harmonic generated by a label-free biological specimen-more precisely collagen fibers forming the connective tissue of a mouse dermis- and discuss of its interpretation. Notably, we show how the SHG phase, qualitatively acting as an indicator of phase-matching conditions, tends to indicate that second harmonic generation, in collagen, is dominated by coherent SHG scattering.
AB - In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional information, inaccessible to incoherent imaging schemes. Last year, we have reported how the SHG phase makes possible real-time nanometric 3D-tracking of SHG emitters, such as nanoparticles (Shaffer et al., Opt. Express 18, p.17392-17403, 2010). Here, we investigate the phase of second harmonic generated by a label-free biological specimen-more precisely collagen fibers forming the connective tissue of a mouse dermis- and discuss of its interpretation. Notably, we show how the SHG phase, qualitatively acting as an indicator of phase-matching conditions, tends to indicate that second harmonic generation, in collagen, is dominated by coherent SHG scattering.
KW - Digital holographic microscopy (DHM)
KW - Holographic SHG imaging
KW - Multiphoton microscopy
KW - Nonlinear phase microscopy
KW - Second harmonic generation (SHG)
UR - http://www.scopus.com/inward/record.url?scp=79955513269&partnerID=8YFLogxK
U2 - 10.1117/12.874538
DO - 10.1117/12.874538
M3 - Conference contribution
AN - SCOPUS:79955513269
SN - 9780819484406
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Multiphoton Microscopy in the Biomedical Sciences XI
T2 - Multiphoton Microscopy in the Biomedical Sciences XI
Y2 - 23 January 2011 through 25 January 2011
ER -