Root cap influences root colonisation by Pseudomonas fluorescens SBW25 on maize

Sonia N. Humphris, A. Glyn Bengough, Bryan S. Griffiths, Ken Kilham, Sheena Rodger, Vicky Stubbs, Tracy A. Valentine, Iain M. Young

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


We investigated the influence of root border cells on the colonisation of seedling Zea mays roots by Pseudomonas fluorescens SBW25 in sandy loam soil packed at two dry bulk densities. Numbers of colony forming units (CFU) were counted on sequential sections of root for intact and decapped inoculated roots grown in loose (1.0 mg m-3) and compacted (1.3 mg m-3) soil. After two days of root growth, the numbers of P. fluorescens (CFU cm -1) were highest on the section of root just below the seed with progressively fewer bacteria near the tip, irrespective of density. The decapped roots had significantly more colonies of P. fluorescens at the tip compared with the intact roots: approximately 100-fold more in the loose and 30-fold more in the compact soil. In addition, confocal images of the root tips grown in agar showed that P. fluorescens could only be detected on the tips of the decapped roots. These results indicated that border cells, and their associated mucilage, prevented complete colonization of the root tip by the biocontrol agent P. fluorescens, possibly by acting as a disposable surface or sheath around the cap. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Original languageEnglish (US)
Pages (from-to)123-130
Number of pages8
JournalFEMS Microbiology Ecology
Issue number1
StatePublished - Sep 1 2005
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-15

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology


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