TY - JOUR
T1 - RNAi silencing of the Arabidopsis thaliana ULCS1 gene results in pleiotropic phenotypes during plant growth and development
AU - Beris, Despoina
AU - Podia, Varvara
AU - Dervisi, Irene
AU - Kapolas, Georgios
AU - Isaioglou, Ioannis
AU - Tsamadou, Vasiliki
AU - Pikoula, Lambrini
AU - Rovoli, Mantalena
AU - Vallianou, Alexandra
AU - Roussis, Andreas
AU - Milioni, Dimitra
AU - Giannoutsou, Helen
AU - Haralampidis, Kosmas
N1 - KAUST Repository Item: Exported on 2022-06-02
PY - 2022
Y1 - 2022
N2 - WD40-repeat-containing proteins (WDRs) are highly abundant in all eukaryotes. Several have been implicated as subunits of multi-protein CRL E3 ligase complexes that regulate ubiquitination mediated protein degradation and thus various cellular and developmental processes. Impairment of the WDR protein ULCS1 from Arabidopsis causes pleiotropic phenotypes during plant development, including reduced lignification, anther indehiscence, and sterility. Here we show that RNAi-mediated downregulation of ULCS1 results in a fast-growing phenotype during vegetative development. Due to accelerated growth, ulcs1i mutants reach their vegetative to reproductive transition point earlier than WT plants. However, their comparable germination rate and their similar number of secondary branches and rosette leaves at bolting indicate that ulcs1i is not an early flowering time mutant. GUS staining of progeny, obtained from crosses between ulcs1i and CYCB1
AB - WD40-repeat-containing proteins (WDRs) are highly abundant in all eukaryotes. Several have been implicated as subunits of multi-protein CRL E3 ligase complexes that regulate ubiquitination mediated protein degradation and thus various cellular and developmental processes. Impairment of the WDR protein ULCS1 from Arabidopsis causes pleiotropic phenotypes during plant development, including reduced lignification, anther indehiscence, and sterility. Here we show that RNAi-mediated downregulation of ULCS1 results in a fast-growing phenotype during vegetative development. Due to accelerated growth, ulcs1i mutants reach their vegetative to reproductive transition point earlier than WT plants. However, their comparable germination rate and their similar number of secondary branches and rosette leaves at bolting indicate that ulcs1i is not an early flowering time mutant. GUS staining of progeny, obtained from crosses between ulcs1i and CYCB1
UR - http://hdl.handle.net/10754/678396
UR - http://www.intjdevbiol.com/paper.php?doi=210114kh
UR - http://www.scopus.com/inward/record.url?scp=85130645144&partnerID=8YFLogxK
U2 - 10.1387/ijdb.210114kh
DO - 10.1387/ijdb.210114kh
M3 - Article
C2 - 34549791
SN - 1696-3547
VL - 66
SP - 177
EP - 186
JO - The International journal of developmental biology
JF - The International journal of developmental biology
IS - 123
ER -