Reprogramming by lineage specifiers: Blurring the lines between pluripotency and differentiation

Ignacio Sancho-Martinez, Alejandro Ocampo, Juan Carlos Izpisua Belmonte*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

6 Scopus citations


The generation of human induced pluripotent stem cells (iPS) has raised enormous expectations within the biomedical community due to their potential vast implications in regenerative and personalized medicine. However, reprogramming to iPS is still not fully comprehended. Difficulties found in ascribing specific molecular patterns to pluripotent cells (PSCs), and inherent inter-line and intra-line variability between different PSCs need to be resolved. Additionally, and despite multiple assumptions, it remains unclear whether the current in vitro culturing conditions for the maintenance and differentiation of PSCs do indeed recapitulate the developmental processes observed in vivo. As a consequence, basic questions such as what is the actual nature of PSCs remain unanswered and different theories have emerged in regards to the identity of these valuable cell population. Here we discuss on the published theories for defining PSC identity, the implications that the different postulated models have for the reprogramming field as well as speculate on potential future directions that might be opened once a precise knowledge on the nature of PSCs is accomplished.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalCurrent Opinion in Genetics and Development
StatePublished - Oct 1 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014 Elsevier Ltd.

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology


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