Relationships between serotypes and genotypes of hepatitis B virus: genetic classification of HBV by use of surface genes

Ken ichi Ohba; Masashi Mizokami; Tomoyoshi Ohno; Kaoru Suzuki; Etsuro Orito; Johnson Y.N. Lau; Yasuo Ina; Kazuho Ikeo; Takashi Gojobori

doi:10.1016/S0168-1702(95)00069-0

Relationships between serotypes and genotypes of hepatitis B virus: genetic classification of HBV by use of surface genes

Ken ichi Ohba, Masashi Mizokami^*, Tomoyoshi Ohno, Kaoru Suzuki, Etsuro Orito, Johnson Y.N. Lau, Yasuo Ina, Kazuho Ikeo, Takashi Gojobori

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Hepatitis B virus (HBV) surface antigen (HBsAg), which is encoded by the HBV S gene, is conventionally classified into 4 serological subtypes, adw, adr, ayw and ayr. To determine the relationship between the HBsAg seroreactivity and the nucleotide sequence diversity of the HBV S gene, the nucleotide sequences of S genes for HBV isolates reported so far were aligned with each other. The numbers of nucleotide substitutions were then estimated by the 6-parameter method, and a phylogenetic tree was constructed by the unweighted paired grouping method with arithmetic mean (UPGMA) and the neighbor-joining (NJ) method. The phylogenetic trees constructed showed that all isolates were grouped into 4 genotypes (gyw, gdw-1, gdw-2, and gdr). More importantly, the genotypes did not necessarily correspond to the conventional serotypes. In particular, serotype 'adw' can be any of genotypes gdw-1, gdw-2, or gdr. Thus, genotyping by S genes gives more accurate information about genetic variation of HBV.

Original language	English (US)
Pages (from-to)	25-34
Number of pages	10
Journal	Virus research
Volume	39
Issue number	1
DOIs	https://doi.org/10.1016/S0168-1702(95)00069-0
State	Published - Nov 1995
Externally published	Yes

Bibliographical note

Funding Information:
authors are also grateful to the DNA Data Bank of Japan (DDBJ) for providing the computing facilities and the integrated DNA database of DDBJ/GenBank/ EMBL. M.M. was supported by grants from the Japanese Ministry of Health and Welfare, Health Science Research Grants (Non-A, non-B Hepatitis Research Grants) and Viral Hepatitis Research Foundation of Japan. J.Y.N.L. was supported in part by the DSR-RDA-I-15 grants from the Division of Sponsored Research, University of Florida, GainesviUe, FL, USA; American Association Hans Popper Liver Scholar Award; and the Glaxo Institute of Digestive Health Clinical Investigator Award. T.G. was supported by the Ministry of Education, Science, and Culture of Japan.

Keywords

Genotype
Hepatitis B virus (HBV)
Molecular evolution
Phylogenetic tree
Subtype

ASJC Scopus subject areas

Virology
Infectious Diseases
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0168-1702(95)00069-0

Cite this

@article{5713a426e4284c79be9cbd456068fbca,

title = "Relationships between serotypes and genotypes of hepatitis B virus: genetic classification of HBV by use of surface genes",

abstract = "Hepatitis B virus (HBV) surface antigen (HBsAg), which is encoded by the HBV S gene, is conventionally classified into 4 serological subtypes, adw, adr, ayw and ayr. To determine the relationship between the HBsAg seroreactivity and the nucleotide sequence diversity of the HBV S gene, the nucleotide sequences of S genes for HBV isolates reported so far were aligned with each other. The numbers of nucleotide substitutions were then estimated by the 6-parameter method, and a phylogenetic tree was constructed by the unweighted paired grouping method with arithmetic mean (UPGMA) and the neighbor-joining (NJ) method. The phylogenetic trees constructed showed that all isolates were grouped into 4 genotypes (gyw, gdw-1, gdw-2, and gdr). More importantly, the genotypes did not necessarily correspond to the conventional serotypes. In particular, serotype 'adw' can be any of genotypes gdw-1, gdw-2, or gdr. Thus, genotyping by S genes gives more accurate information about genetic variation of HBV.",

keywords = "Genotype, Hepatitis B virus (HBV), Molecular evolution, Phylogenetic tree, Subtype",

author = "Ohba, {Ken ichi} and Masashi Mizokami and Tomoyoshi Ohno and Kaoru Suzuki and Etsuro Orito and Lau, {Johnson Y.N.} and Yasuo Ina and Kazuho Ikeo and Takashi Gojobori",

note = "Funding Information: authors are also grateful to the DNA Data Bank of Japan (DDBJ) for providing the computing facilities and the integrated DNA database of DDBJ/GenBank/ EMBL. M.M. was supported by grants from the Japanese Ministry of Health and Welfare, Health Science Research Grants (Non-A, non-B Hepatitis Research Grants) and Viral Hepatitis Research Foundation of Japan. J.Y.N.L. was supported in part by the DSR-RDA-I-15 grants from the Division of Sponsored Research, University of Florida, GainesviUe, FL, USA; American Association Hans Popper Liver Scholar Award; and the Glaxo Institute of Digestive Health Clinical Investigator Award. T.G. was supported by the Ministry of Education, Science, and Culture of Japan.",

year = "1995",

month = nov,

doi = "10.1016/S0168-1702(95)00069-0",

language = "English (US)",

volume = "39",

pages = "25--34",

journal = "Virus research",

issn = "0168-1702",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Relationships between serotypes and genotypes of hepatitis B virus

T2 - genetic classification of HBV by use of surface genes

AU - Ohba, Ken ichi

AU - Mizokami, Masashi

AU - Ohno, Tomoyoshi

AU - Suzuki, Kaoru

AU - Orito, Etsuro

AU - Lau, Johnson Y.N.

AU - Ina, Yasuo

AU - Ikeo, Kazuho

AU - Gojobori, Takashi

N1 - Funding Information: authors are also grateful to the DNA Data Bank of Japan (DDBJ) for providing the computing facilities and the integrated DNA database of DDBJ/GenBank/ EMBL. M.M. was supported by grants from the Japanese Ministry of Health and Welfare, Health Science Research Grants (Non-A, non-B Hepatitis Research Grants) and Viral Hepatitis Research Foundation of Japan. J.Y.N.L. was supported in part by the DSR-RDA-I-15 grants from the Division of Sponsored Research, University of Florida, GainesviUe, FL, USA; American Association Hans Popper Liver Scholar Award; and the Glaxo Institute of Digestive Health Clinical Investigator Award. T.G. was supported by the Ministry of Education, Science, and Culture of Japan.

PY - 1995/11

Y1 - 1995/11

N2 - Hepatitis B virus (HBV) surface antigen (HBsAg), which is encoded by the HBV S gene, is conventionally classified into 4 serological subtypes, adw, adr, ayw and ayr. To determine the relationship between the HBsAg seroreactivity and the nucleotide sequence diversity of the HBV S gene, the nucleotide sequences of S genes for HBV isolates reported so far were aligned with each other. The numbers of nucleotide substitutions were then estimated by the 6-parameter method, and a phylogenetic tree was constructed by the unweighted paired grouping method with arithmetic mean (UPGMA) and the neighbor-joining (NJ) method. The phylogenetic trees constructed showed that all isolates were grouped into 4 genotypes (gyw, gdw-1, gdw-2, and gdr). More importantly, the genotypes did not necessarily correspond to the conventional serotypes. In particular, serotype 'adw' can be any of genotypes gdw-1, gdw-2, or gdr. Thus, genotyping by S genes gives more accurate information about genetic variation of HBV.

AB - Hepatitis B virus (HBV) surface antigen (HBsAg), which is encoded by the HBV S gene, is conventionally classified into 4 serological subtypes, adw, adr, ayw and ayr. To determine the relationship between the HBsAg seroreactivity and the nucleotide sequence diversity of the HBV S gene, the nucleotide sequences of S genes for HBV isolates reported so far were aligned with each other. The numbers of nucleotide substitutions were then estimated by the 6-parameter method, and a phylogenetic tree was constructed by the unweighted paired grouping method with arithmetic mean (UPGMA) and the neighbor-joining (NJ) method. The phylogenetic trees constructed showed that all isolates were grouped into 4 genotypes (gyw, gdw-1, gdw-2, and gdr). More importantly, the genotypes did not necessarily correspond to the conventional serotypes. In particular, serotype 'adw' can be any of genotypes gdw-1, gdw-2, or gdr. Thus, genotyping by S genes gives more accurate information about genetic variation of HBV.

KW - Genotype

KW - Hepatitis B virus (HBV)

KW - Molecular evolution

KW - Phylogenetic tree

KW - Subtype

UR - http://www.scopus.com/inward/record.url?scp=0028973455&partnerID=8YFLogxK

U2 - 10.1016/S0168-1702(95)00069-0

DO - 10.1016/S0168-1702(95)00069-0

M3 - Article

C2 - 8607280

AN - SCOPUS:0028973455

SN - 0168-1702

VL - 39

SP - 25

EP - 34

JO - Virus research

JF - Virus research

IS - 1

ER -

Relationships between serotypes and genotypes of hepatitis B virus: genetic classification of HBV by use of surface genes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this