Regeneration and reprogramming compared

Bea Christen, Vanesa Robles, Marina Raya, Ida Paramonov, Juan C.I. Belmonte*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Background: Dedifferentiation occurs naturally in mature cell types during epimorphic regeneration in fish and some amphibians. Dedifferentiation also occurs in the induction of pluripotent stem cells when a set of transcription factors (Oct4, Sox2, Klf4 and c-Myc) is over expressed in mature cell types.Results: We hypothesised that there are parallels between dedifferentiation or reprogramming of somatic cells to induced pluripotent stem cells and the natural process of dedifferentiation during epimorphic regeneration. We analysed expression levels of the most commonly used pluripotency associated factors in regenerating and non-regenerating tissue and compared them with levels in a pluripotent reference cell. We found that some of the pluripotency associated factors (oct4/pou5f1, sox2, c-myc, klf4, tert, sall4, zic3, dppa2/4 and fut1, a homologue of ssea1) were expressed before and during regeneration and that at least two of these factors (oct4, sox2) were also required for normal fin regeneration in the zebrafish. However these factors were not upregulated during regeneration as would be expected if blastema cells acquired pluripotency.Conclusions: By comparing cells from the regeneration blastema with embryonic pluripotent reference cells we found that induced pluripotent stem and blastema cells do not share pluripotency. However, during blastema formation some of the key reprogramming factors are both expressed and are also required for regeneration to take place. We therefore propose a link between partially reprogrammed induced pluripotent stem cells and the half way state of blastema cells and suggest that a common mechanism might be regulating these two processes.

Original languageEnglish (US)
Article number5
JournalBMC Biology
Volume8
DOIs
StatePublished - Jan 20 2010

Bibliographical note

Funding Information:
We thank Carme Fabregat, Dr. Mercé Martí, Dr. Filipe Martinez-Pastor, José Miguel Vaquero, Lola Mulero, Cristina Pardo and Cristina Morera for technical assistance. This work was partially supported by grants from Ramón y Cajal Program RYC-2008-02339 (VR), Fondo de Investigaciones Sanitarias (TERCEL, PI052847), Marató de TV3, MICINN, Fundación Cellex, The G. Harold and Leila Y. Mathers Charitable Foundation and Ipsen Foundation.

ASJC Scopus subject areas

  • Biotechnology
  • Structural Biology
  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Plant Science
  • Developmental Biology
  • Cell Biology

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