Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 105, thus revealing details down to few molecules resolution.
Bibliographical noteFunding Information:
Acknowledgments The authors gratefully acknowledge support from European Projects SMD FP7 no. CP-FP 229375-2; Nanoantenna FP7 no. 241818; FOCUS FP7 no. 270483.
- Amyloid fibrils
- Field enhancement
- Raman spectroscopy
- Top-illumination setup
ASJC Scopus subject areas