Red blood cell structure and dynamics explored with digital holographic microscopy

P. Marquet*, B. Rappaz, A. Barbuld, R. Korensteind, C. Depeursinge, P. Magistretti

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations


Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. Thanks to a decoupling procedure cell thickness and intracellular refractive index can be measured separately. Consequently, Mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), two highly relevant clinical parameters, have been measured non-invasively at a single cell level. The DHM nanometric axial and microsecond temporal sensitivities have permitted to measure the red blood cell membrane fluctuations (CMF) on the whole cell surface.

Original languageEnglish (US)
Article number71821A
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2009
Externally publishedYes
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII - San Jose, CA, United States
Duration: Jan 26 2009Jan 28 2009


  • Cell imaging
  • Digital holography
  • Erythrocytec
  • Refractive index

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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