Reconstruction from serial sections: A tool for developmental biology. Application to Hox genes expression in chicken wing buds

J. ‐C Olivo; J. ‐C Izpisúa‐Belmonte; C. Tickle; C. Boulin; D. Duboule

doi:10.1002/1361-6374(199309)1:3<151::AID-BIO3>3.3.CO;2-E

Reconstruction from serial sections: A tool for developmental biology. Application to Hox genes expression in chicken wing buds

J. ‐C Olivo^*, J. ‐C Izpisúa‐Belmonte, C. Tickle, C. Boulin, D. Duboule

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A method for analysing the localization of the Hox‐4 gene complex by three‐dimensional reconstruction of serial cross‐sections is presented. It allows the simultaneous visualization of three Hox expression domains on the same reconstructed embryo and the study of the effect of a graft of chicken polarizing region on the de novo expression of the genes of the HOX‐4 complex. The method consists of registering the sections, segmenting the biological patterns and finally displaying the reconstructed embryo. The precise relationships between the transcript domains of the three genes can thus be assessed in either the normal wing bud or upon pattern modification.

Original language	English (US)
Pages (from-to)	151-158
Number of pages	8
Journal	Bioimaging
Volume	1
Issue number	3
DOIs	https://doi.org/10.1002/1361-6374(199309)1:3<151::AID-BIO3>3.3.CO;2-E
State	Published - Sep 1993
Externally published	Yes

Keywords

3‐D reconstruction
Hox genes
developmental biology
registration
serial sections

ASJC Scopus subject areas

Biophysics
Radiology Nuclear Medicine and imaging

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10.1002/1361-6374(199309)1:3<151::AID-BIO3>3.3.CO;2-E

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title = "Reconstruction from serial sections: A tool for developmental biology. Application to Hox genes expression in chicken wing buds",

abstract = "A method for analysing the localization of the Hox‐4 gene complex by three‐dimensional reconstruction of serial cross‐sections is presented. It allows the simultaneous visualization of three Hox expression domains on the same reconstructed embryo and the study of the effect of a graft of chicken polarizing region on the de novo expression of the genes of the HOX‐4 complex. The method consists of registering the sections, segmenting the biological patterns and finally displaying the reconstructed embryo. The precise relationships between the transcript domains of the three genes can thus be assessed in either the normal wing bud or upon pattern modification.",

keywords = "3‐D reconstruction, Hox genes, developmental biology, registration, serial sections",

author = "Olivo, {J. ‐C} and Izpis{\'u}a‐Belmonte, {J. ‐C} and C. Tickle and C. Boulin and D. Duboule",

year = "1993",

month = sep,

doi = "10.1002/1361-6374(199309)1:3<151::AID-BIO3>3.3.CO;2-E",

language = "English (US)",

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journal = "Bioimaging",

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TY - JOUR

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T2 - A tool for developmental biology. Application to Hox genes expression in chicken wing buds

AU - Olivo, J. ‐C

AU - Izpisúa‐Belmonte, J. ‐C

AU - Tickle, C.

AU - Boulin, C.

AU - Duboule, D.

PY - 1993/9

Y1 - 1993/9

N2 - A method for analysing the localization of the Hox‐4 gene complex by three‐dimensional reconstruction of serial cross‐sections is presented. It allows the simultaneous visualization of three Hox expression domains on the same reconstructed embryo and the study of the effect of a graft of chicken polarizing region on the de novo expression of the genes of the HOX‐4 complex. The method consists of registering the sections, segmenting the biological patterns and finally displaying the reconstructed embryo. The precise relationships between the transcript domains of the three genes can thus be assessed in either the normal wing bud or upon pattern modification.

AB - A method for analysing the localization of the Hox‐4 gene complex by three‐dimensional reconstruction of serial cross‐sections is presented. It allows the simultaneous visualization of three Hox expression domains on the same reconstructed embryo and the study of the effect of a graft of chicken polarizing region on the de novo expression of the genes of the HOX‐4 complex. The method consists of registering the sections, segmenting the biological patterns and finally displaying the reconstructed embryo. The precise relationships between the transcript domains of the three genes can thus be assessed in either the normal wing bud or upon pattern modification.

KW - 3‐D reconstruction

KW - Hox genes

KW - developmental biology

KW - registration

KW - serial sections

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DO - 10.1002/1361-6374(199309)1:3<151::AID-BIO3>3.3.CO;2-E

M3 - Article

AN - SCOPUS:84982484980

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JO - Bioimaging

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Reconstruction from serial sections: A tool for developmental biology. Application to Hox genes expression in chicken wing buds

Abstract

Keywords

ASJC Scopus subject areas

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