Rapid identification of rust resistance genes through cultivar-specific de novo chromosome assemblies

Anupriya K. Thind; Thomas Wicker; Simon G. Krattinger

doi:10.1007/978-1-4939-7249-4_21

Rapid identification of rust resistance genes through cultivar-specific de novo chromosome assemblies

Anupriya K. Thind, Thomas Wicker, Simon G. Krattinger^*

^*Corresponding author for this work

Plant Science

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus citations

Abstract

“Map-based cloning” is a frequently used approach to isolate rust resistance genes. A critical step during map-based cloning is the transition from genetic information, i.e., a genetic map, to physical sequence information. Bacterial artificial chromosome clones are often used to establish sequence information spanning a genetic interval. However, a major limitation of BAC clones consists in their small insert size of 100–200 kb. Targeted chromosome-based cloning via long-range assembly (TACCA) is a method that can replace BAC library screening. This approach involves chromosome flow-sorting and the establishment of a long-range de novo assembly. This chapter provides an overview of TACCA as well as a detailed description of sequence analyses, molecular marker development, and candidate gene identification.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	245-255
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-7249-4_21
State	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1659
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media LLC 2017.

Keywords

Candidate gene
Chromosome flow-sorting
Long-range scaffolding
Map-based cloning
Molecular marker

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-7249-4_21

Cite this

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title = "Rapid identification of rust resistance genes through cultivar-specific de novo chromosome assemblies",

abstract = "“Map-based cloning” is a frequently used approach to isolate rust resistance genes. A critical step during map-based cloning is the transition from genetic information, i.e., a genetic map, to physical sequence information. Bacterial artificial chromosome clones are often used to establish sequence information spanning a genetic interval. However, a major limitation of BAC clones consists in their small insert size of 100–200 kb. Targeted chromosome-based cloning via long-range assembly (TACCA) is a method that can replace BAC library screening. This approach involves chromosome flow-sorting and the establishment of a long-range de novo assembly. This chapter provides an overview of TACCA as well as a detailed description of sequence analyses, molecular marker development, and candidate gene identification.",

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AB - “Map-based cloning” is a frequently used approach to isolate rust resistance genes. A critical step during map-based cloning is the transition from genetic information, i.e., a genetic map, to physical sequence information. Bacterial artificial chromosome clones are often used to establish sequence information spanning a genetic interval. However, a major limitation of BAC clones consists in their small insert size of 100–200 kb. Targeted chromosome-based cloning via long-range assembly (TACCA) is a method that can replace BAC library screening. This approach involves chromosome flow-sorting and the establishment of a long-range de novo assembly. This chapter provides an overview of TACCA as well as a detailed description of sequence analyses, molecular marker development, and candidate gene identification.

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Rapid identification of rust resistance genes through cultivar-specific de novo chromosome assemblies

Abstract

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Keywords

ASJC Scopus subject areas

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