Rapid gene isolation using MutChromSeq

Burkhard Steuernagel; Jan Vrána; Miroslava Karafiátová; Brande B.H. Wulff; Jaroslav Doležel

doi:10.1007/978-1-4939-7249-4_20

Rapid gene isolation using MutChromSeq

Burkhard Steuernagel, Jan Vrána, Miroslava Karafiátová, Brande B.H. Wulff, Jaroslav Doležel

Research output: Chapter in Book/Report/Conference proceeding › Chapter

12 Scopus citations

Abstract

MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press [email protected]
Pages	231-243
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-4939-7249-4_20
State	Published - Jan 1 2017
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-20

ASJC Scopus subject areas

Genetics
Molecular Biology

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10.1007/978-1-4939-7249-4_20

Cite this

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abstract = "MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.",

author = "Burkhard Steuernagel and Jan Vr{\'a}na and Miroslava Karafi{\'a}tov{\'a} and Wulff, {Brande B.H.} and Jaroslav Dole{\v z}el",

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doi = "10.1007/978-1-4939-7249-4_20",

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AU - Steuernagel, Burkhard

AU - Vrána, Jan

AU - Karafiátová, Miroslava

AU - Wulff, Brande B.H.

AU - Doležel, Jaroslav

N1 - Generated from Scopus record by KAUST IRTS on 2023-02-20

PY - 2017/1/1

Y1 - 2017/1/1

N2 - MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.

AB - MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.

UR - http://link.springer.com/10.1007/978-1-4939-7249-4_20

UR - http://www.scopus.com/inward/record.url?scp=85028985949&partnerID=8YFLogxK

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DO - 10.1007/978-1-4939-7249-4_20

M3 - Chapter

SP - 231

EP - 243

BT - Methods in Molecular Biology

PB - Humana Press [email protected]

ER -

Rapid gene isolation using MutChromSeq

Abstract

Bibliographical note

ASJC Scopus subject areas

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