Rapid gene isolation using MutChromSeq

Burkhard Steuernagel, Jan Vrána, Miroslava Karafiátová, Brande B.H. Wulff, Jaroslav Doležel

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.
Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.humana@humanapr.com
Pages231-243
Number of pages13
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-20

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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