Rapid gene isolation in barley and wheat by mutant chromosome sequencing

Javier Sánchez-Martín; Burkhard Steuernagel; Sreya Ghosh; Gerhard Herren; Severine Hurni; Nikolai Adamski; Jan Vrána; Marie Kubaláková; Simon G. Krattinger; Thomas Wicker; Jaroslav Doležel; Beat Keller; Brande B.H. Wulff

doi:10.1186/s13059-016-1082-1

Rapid gene isolation in barley and wheat by mutant chromosome sequencing

Javier Sánchez-Martín, Burkhard Steuernagel, Sreya Ghosh, Gerhard Herren, Severine Hurni, Nikolai Adamski, Jan Vrána, Marie Kubaláková, Simon G. Krattinger, Thomas Wicker, Jaroslav Doležel, Beat Keller, Brande B.H. Wulff

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251 Scopus citations

Abstract

Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.

Original language	English (US)
Journal	Genome biology
Volume	17
Issue number	1
DOIs	https://doi.org/10.1186/s13059-016-1082-1
State	Published - Oct 31 2016
Externally published	Yes

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title = "Rapid gene isolation in barley and wheat by mutant chromosome sequencing",

abstract = "Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.",

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T1 - Rapid gene isolation in barley and wheat by mutant chromosome sequencing

AU - Sánchez-Martín, Javier

AU - Steuernagel, Burkhard

AU - Ghosh, Sreya

AU - Herren, Gerhard

AU - Hurni, Severine

AU - Adamski, Nikolai

AU - Vrána, Jan

AU - Kubaláková, Marie

AU - Krattinger, Simon G.

AU - Wicker, Thomas

AU - Doležel, Jaroslav

AU - Keller, Beat

AU - Wulff, Brande B.H.

N1 - Generated from Scopus record by KAUST IRTS on 2023-02-20

PY - 2016/10/31

Y1 - 2016/10/31

N2 - Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.

AB - Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.

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JO - Genome biology

JF - Genome biology

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ER -

Rapid gene isolation in barley and wheat by mutant chromosome sequencing

Abstract

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