Rapid Gene Cloning in Wheat

M. Asyraf Md Hatta, Burkhard Steuernagel, Brande B.H. Wulff

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations

Abstract

The identification of wheat and barley genes controlling important agronomic traits using positional cloning has traditionally been a challenging and time-consuming procedure. This is due to the enormous genome size and high repeat content from transposable elements (TEs). Low marker density, suppressed recombination, and the high cost of generating a physical contig across a genetically defined map interval have further restricted the application of positional approximation. Over the past decade, the cost of DNA sequencing has significantly dropped, as has our ability to computationally analyze large quantities of DNA sequence data. This has enabled researchers to exploit next-generation sequencing (NGS) technologies more routinely to accelerate the gene cloning process. In this chapter, we discuss several newly emerging cloning methods that combine NGS technologies with recent advances in molecular genomics to overcome previous limitations of gene cloning in wheat and barley.
Original languageEnglish (US)
Title of host publicationApplications of Genetic and Genomic Research in Cereals
PublisherElsevier
Pages65-95
Number of pages31
ISBN (Print)9780081021637
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-20

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