Rapid enzymatic hydrolysis of crambe oil catalyzed by castor seeds lipases

F. Tavares, P. R. Sackser, C. E. Borba, E. A. Silva

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Oil hydrolysis is an important industrial process that requires high temperatures and pressure, or expensive enzymatic catalysts; it is, therefore, necessary to seek the use of inexpensive raw materials and process enhancement. In this work, a rapid and eco-friendly method, using ultrasound power, was successfully employed to hydrolyze crambe (Crambe abyssinica Hochst.) oil using lipase enzymes directly from castor (Ricinus communis L.) seeds, in oil-free and fresh forms. A yield of 86 % conversion of triglycerides into free fatty acids (FFA) was achieved in 5 min using castor fresh seeds and 73 % of conversion when performing the reaction with oil-free seeds. The operational conditions of ultrasound power, mass ratio of buffer solution and oil, catalyst, and total substrate were evaluated using a central composite rotatable design (CCRD). The hydrolysis yield was optimized by response surface methodology (RSM). The optimum conditions were approximately 70 % of ultrasound power (350 W), 1.79 buffer solution/oil mass ratio, and 0.25 catalyst/substrate mass ratio for fresh seeds. For the oil-free seeds, the optimal conditions found were 68 % (340 W) of ultrasound power, 1.67 buffer solution/oil mass ratio, and 0.06 catalyst/substrate mass ratio. Mathematical modeling was applied to the experimental kinetic data, and it was possible to predict FFA concentration values from independent experiments.
Original languageEnglish (US)
Pages (from-to)113890
JournalIndustrial Crops and Products
StatePublished - Aug 13 2021
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2021-08-20
Acknowledgements: This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The authors would like to thank Professor Himanshu Mishra from King Abdullah University of Science and Technology (KAUST), for the possibility of the NMR analysis.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

ASJC Scopus subject areas

  • Agronomy and Crop Science


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