TY - JOUR
T1 - Raman on suspended DNA: Novel super-hydrophobic approach for structural studies
AU - Marini, Monica
AU - Allione, Marco
AU - Torre, Bruno
AU - Moretti, Manola
AU - Limongi, Tania
AU - Tirinato, Luca
AU - Giugni, Andrea
AU - Das, Gobind
AU - Di Fabrizio, Enzo M.
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors acknowledge financial support from the KAUST start-up funding and from Ministry of Health, Italy under the projects: Project no.: GR-2010-2320665 and Project no.: GR-2010-2311677.
PY - 2016/12/24
Y1 - 2016/12/24
N2 - The A- and B-form are two of the most common structural conformations of double strand DNA present in nature and they can interchange on the basis of the helices hydration [1,2]. Herein we demonstrate that the use of non-destructive techniques such as Raman spectroscopy coupled with the use of a super-hydrophobic device, allows the clear identification of the DNA hydration state, of the backbone (phosphate + deoxyribose sugar) conformation and of the nucleotides. There is a wide prospect for an increase of knowledge in biomolecules using this combined approach resulting in a significant impact in the study of more complex supramolecular assemblies and of fine chemical variation along the genomic loci undergoing to epigenetic variations.
AB - The A- and B-form are two of the most common structural conformations of double strand DNA present in nature and they can interchange on the basis of the helices hydration [1,2]. Herein we demonstrate that the use of non-destructive techniques such as Raman spectroscopy coupled with the use of a super-hydrophobic device, allows the clear identification of the DNA hydration state, of the backbone (phosphate + deoxyribose sugar) conformation and of the nucleotides. There is a wide prospect for an increase of knowledge in biomolecules using this combined approach resulting in a significant impact in the study of more complex supramolecular assemblies and of fine chemical variation along the genomic loci undergoing to epigenetic variations.
UR - http://hdl.handle.net/10754/622075
UR - http://www.sciencedirect.com/science/article/pii/S0167931716305275
UR - http://www.scopus.com/inward/record.url?scp=85008462680&partnerID=8YFLogxK
U2 - 10.1016/j.mee.2016.12.016
DO - 10.1016/j.mee.2016.12.016
M3 - Article
SN - 0167-9317
VL - 175
SP - 38
EP - 42
JO - Microelectronic Engineering
JF - Microelectronic Engineering
ER -