Raman database of amino acids solutions: A critical study of Extended Multiplicative Signal Correction

Patrizio Candeloro; Elisabetta Grande; Raffaella Raimondo; Daniele Di Mascolo; Francesco T. Gentile; Maria Laura Coluccio; Gerardo Perozziello; Natalia Maria Malara; Marco Francardi; Enzo M. Di Fabrizio

doi:10.1039/c3an01665j

Raman database of amino acids solutions: A critical study of Extended Multiplicative Signal Correction

Patrizio Candeloro, Elisabetta Grande, Raffaella Raimondo, Daniele Di Mascolo, Francesco T. Gentile, Maria Laura Coluccio, Gerardo Perozziello, Natalia Maria Malara, Marco Francardi, Enzo M. Di Fabrizio

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43 Scopus citations

Abstract

The Raman spectra of biological materials always exhibit complex profiles, constituting several peaks and/or bands which arise due to the large variety of biomolecules. The extraction of quantitative information from these spectra is not a trivial task. While qualitative information can be retrieved from the changes in peaks frequencies or from the appearance/disappearance of some peaks, quantitative analysis requires an examination of peak intensities. Unfortunately in biological samples it is not easy to identify a reference peak for normalizing intensities, and this makes it very difficult to study the peak intensities. In the last decades a more refined mathematical tool, the extended multiplicative signal correction (EMSC), has been proposed for treating infrared spectra, which is also capable of providing quantitative information. From the mathematical and physical point of view, EMSC can also be applied to Raman spectra, as recently proposed. In this work the reliability of the EMSC procedure is tested by application to a well defined biological system: the 20 standard amino acids and their combination in peptides. The first step is the collection of a Raman database of these 20 amino acids, and subsequently EMSC processing is applied to retrieve quantitative information from amino acids mixtures and peptides. A critical review of the results is presented, showing that EMSC has to be carefully handled for complex biological systems. © 2013 The Royal Society of Chemistry.

Original language	English (US)
Pages (from-to)	7331
Journal	Analyst
Volume	138
Issue number	24
DOIs	https://doi.org/10.1039/c3an01665j
State	Published - 2013

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors gratefully acknowledge Dr Camillo Palmieri for providing the peptides IBTK1 and IBTK2. Financial support is acknowledged from Italian-FIRB project "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod. RBPR05JH2P_010, CUP B41J09000110005) and from Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338).

ASJC Scopus subject areas

Biochemistry
Spectroscopy
Environmental Chemistry
Analytical Chemistry
Electrochemistry

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@article{5a702fee7c0149f99ef3b727fda48dad,

title = "Raman database of amino acids solutions: A critical study of Extended Multiplicative Signal Correction",

abstract = "The Raman spectra of biological materials always exhibit complex profiles, constituting several peaks and/or bands which arise due to the large variety of biomolecules. The extraction of quantitative information from these spectra is not a trivial task. While qualitative information can be retrieved from the changes in peaks frequencies or from the appearance/disappearance of some peaks, quantitative analysis requires an examination of peak intensities. Unfortunately in biological samples it is not easy to identify a reference peak for normalizing intensities, and this makes it very difficult to study the peak intensities. In the last decades a more refined mathematical tool, the extended multiplicative signal correction (EMSC), has been proposed for treating infrared spectra, which is also capable of providing quantitative information. From the mathematical and physical point of view, EMSC can also be applied to Raman spectra, as recently proposed. In this work the reliability of the EMSC procedure is tested by application to a well defined biological system: the 20 standard amino acids and their combination in peptides. The first step is the collection of a Raman database of these 20 amino acids, and subsequently EMSC processing is applied to retrieve quantitative information from amino acids mixtures and peptides. A critical review of the results is presented, showing that EMSC has to be carefully handled for complex biological systems. {\textcopyright} 2013 The Royal Society of Chemistry.",

author = "Patrizio Candeloro and Elisabetta Grande and Raffaella Raimondo and {Di Mascolo}, Daniele and Gentile, {Francesco T.} and Coluccio, {Maria Laura} and Gerardo Perozziello and Malara, {Natalia Maria} and Marco Francardi and {Di Fabrizio}, {Enzo M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors gratefully acknowledge Dr Camillo Palmieri for providing the peptides IBTK1 and IBTK2. Financial support is acknowledged from Italian-FIRB project {"}Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet{"} (cod. RBPR05JH2P_010, CUP B41J09000110005) and from Cariplo Foundation under the project {"}New Frontiers in Plasmonic Nanosensing{"} (Grant no. 2011-0338).",

year = "2013",

doi = "10.1039/c3an01665j",

language = "English (US)",

volume = "138",

pages = "7331",

journal = "Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

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T1 - Raman database of amino acids solutions: A critical study of Extended Multiplicative Signal Correction

AU - Candeloro, Patrizio

AU - Grande, Elisabetta

AU - Raimondo, Raffaella

AU - Di Mascolo, Daniele

AU - Gentile, Francesco T.

AU - Coluccio, Maria Laura

AU - Perozziello, Gerardo

AU - Malara, Natalia Maria

AU - Francardi, Marco

AU - Di Fabrizio, Enzo M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors gratefully acknowledge Dr Camillo Palmieri for providing the peptides IBTK1 and IBTK2. Financial support is acknowledged from Italian-FIRB project "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod. RBPR05JH2P_010, CUP B41J09000110005) and from Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338).

PY - 2013

Y1 - 2013

N2 - The Raman spectra of biological materials always exhibit complex profiles, constituting several peaks and/or bands which arise due to the large variety of biomolecules. The extraction of quantitative information from these spectra is not a trivial task. While qualitative information can be retrieved from the changes in peaks frequencies or from the appearance/disappearance of some peaks, quantitative analysis requires an examination of peak intensities. Unfortunately in biological samples it is not easy to identify a reference peak for normalizing intensities, and this makes it very difficult to study the peak intensities. In the last decades a more refined mathematical tool, the extended multiplicative signal correction (EMSC), has been proposed for treating infrared spectra, which is also capable of providing quantitative information. From the mathematical and physical point of view, EMSC can also be applied to Raman spectra, as recently proposed. In this work the reliability of the EMSC procedure is tested by application to a well defined biological system: the 20 standard amino acids and their combination in peptides. The first step is the collection of a Raman database of these 20 amino acids, and subsequently EMSC processing is applied to retrieve quantitative information from amino acids mixtures and peptides. A critical review of the results is presented, showing that EMSC has to be carefully handled for complex biological systems. © 2013 The Royal Society of Chemistry.

AB - The Raman spectra of biological materials always exhibit complex profiles, constituting several peaks and/or bands which arise due to the large variety of biomolecules. The extraction of quantitative information from these spectra is not a trivial task. While qualitative information can be retrieved from the changes in peaks frequencies or from the appearance/disappearance of some peaks, quantitative analysis requires an examination of peak intensities. Unfortunately in biological samples it is not easy to identify a reference peak for normalizing intensities, and this makes it very difficult to study the peak intensities. In the last decades a more refined mathematical tool, the extended multiplicative signal correction (EMSC), has been proposed for treating infrared spectra, which is also capable of providing quantitative information. From the mathematical and physical point of view, EMSC can also be applied to Raman spectra, as recently proposed. In this work the reliability of the EMSC procedure is tested by application to a well defined biological system: the 20 standard amino acids and their combination in peptides. The first step is the collection of a Raman database of these 20 amino acids, and subsequently EMSC processing is applied to retrieve quantitative information from amino acids mixtures and peptides. A critical review of the results is presented, showing that EMSC has to be carefully handled for complex biological systems. © 2013 The Royal Society of Chemistry.

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UR - http://xlink.rsc.org/?DOI=c3an01665j

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U2 - 10.1039/c3an01665j

DO - 10.1039/c3an01665j

M3 - Article

C2 - 24153318

SN - 0003-2654

VL - 138

SP - 7331

JO - Analyst

JF - Analyst

IS - 24

ER -

Raman database of amino acids solutions: A critical study of Extended Multiplicative Signal Correction

Abstract

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