TY - CHAP
T1 - Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation
AU - Barrios-Llerena, Martin
AU - Le Bihan, Thierry
N1 - KAUST Repository Item: Exported on 2020-10-01
PY - 2019/4/13
Y1 - 2019/4/13
N2 - Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.
AB - Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.
UR - http://hdl.handle.net/10754/653039
UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-9232-4_4
UR - http://www.scopus.com/inward/record.url?scp=85064741586&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9232-4_4
DO - 10.1007/978-1-4939-9232-4_4
M3 - Chapter
C2 - 30980321
SN - 9781493992317
SP - 35
EP - 42
BT - Mass Spectrometry of Proteins
PB - Springer Nature
ER -