Quantitative phase imaging in biomedicine

YongKeun Park, Christian Depeursinge, Gabriel Popescu

Research output: Contribution to journalArticlepeer-review

995 Scopus citations


Quantitative phase imaging (QPI) has emerged as a valuable method for investigating cells and tissues. QPI operates on unlabelled specimens and, as such, is complementary to established fluorescence microscopy, exhibiting lower phototoxicity and no photobleaching. As the images represent quantitative maps of optical path length delays introduced by the specimen, QPI provides an objective measure of morphology and dynamics, free of variability due to contrast agents. Owing to the tremendous progress witnessed especially in the past 10–15 years, a number of technologies have become sufficiently reliable and translated to biomedical laboratories. Commercialization efforts are under way and, as a result, the QPI field is now transitioning from a technology-development-driven to an application-focused field. In this Review, we aim to provide a critical and objective overview of this dynamic research field by presenting the scientific context, main principles of operation and current biomedical applications.Over the past 10–15 years, quantitative phase imaging has moved from a research-driven to an application-focused field. This Review presents the main principles of operation and representative basic and clinical science applications.
Original languageEnglish (US)
Pages (from-to)578-589
Number of pages12
JournalNature Photonics
Issue number10
StatePublished - Sep 27 2018

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: Y.K.P. was supported by the National Research Foundation of Korea (2017M3C1A3013923, 2015R1A3A2066550, 2017K000396). G.P. was supported by the National Science Foundation (STC CBET 0939511, NSF BRAIN EAGER DBI 1450962, IIP-1353368).


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