Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing

Congli Wang; Qiang Fu; Xiong Dun; Wolfgang Heidrich

doi:10.1038/s41598-019-50264-3

Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing

Congli Wang, Qiang Fu, Xiong Dun, Wolfgang Heidrich

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate and expensive quantitative methods. Here we demonstrate a low-cost, easy to implement microscopy setup for quantitative imaging of phase and bright field amplitude using collimated white light illumination.

Original language	English (US)
Journal	Scientific Reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-019-50264-3
State	Published - Sep 24 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors thank Dr. Fathia Ben Rached, Ioannis Isaioglou, Shahad Alsaiari, and Michael Margineanu for their help in preparing the biological specimens. This work was supported by King Abdullah University of Science and Technology Individual Baseline Funding, as well as Center Partnership Funding.

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10.1038/s41598-019-50264-3

https://repository.kaust.edu.sa/bitstream/10754/656921/1/s41598-019-50264-3.pdf

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title = "Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing",

abstract = "Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate and expensive quantitative methods. Here we demonstrate a low-cost, easy to implement microscopy setup for quantitative imaging of phase and bright field amplitude using collimated white light illumination.",

author = "Congli Wang and Qiang Fu and Xiong Dun and Wolfgang Heidrich",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors thank Dr. Fathia Ben Rached, Ioannis Isaioglou, Shahad Alsaiari, and Michael Margineanu for their help in preparing the biological specimens. This work was supported by King Abdullah University of Science and Technology Individual Baseline Funding, as well as Center Partnership Funding.",

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doi = "10.1038/s41598-019-50264-3",

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T1 - Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing

AU - Wang, Congli

AU - Fu, Qiang

AU - Dun, Xiong

AU - Heidrich, Wolfgang

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors thank Dr. Fathia Ben Rached, Ioannis Isaioglou, Shahad Alsaiari, and Michael Margineanu for their help in preparing the biological specimens. This work was supported by King Abdullah University of Science and Technology Individual Baseline Funding, as well as Center Partnership Funding.

PY - 2019/9/24

Y1 - 2019/9/24

N2 - Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate and expensive quantitative methods. Here we demonstrate a low-cost, easy to implement microscopy setup for quantitative imaging of phase and bright field amplitude using collimated white light illumination.

AB - Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate and expensive quantitative methods. Here we demonstrate a low-cost, easy to implement microscopy setup for quantitative imaging of phase and bright field amplitude using collimated white light illumination.

UR - http://hdl.handle.net/10754/656921

UR - http://www.nature.com/articles/s41598-019-50264-3

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SN - 2045-2322

VL - 9

JO - Scientific Reports

JF - Scientific Reports

IS - 1

ER -

Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing

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