Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate and expensive quantitative methods. Here we demonstrate a low-cost, easy to implement microscopy setup for quantitative imaging of phase and bright field amplitude using collimated white light illumination.
Bibliographical noteKAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors thank Dr. Fathia Ben Rached, Ioannis Isaioglou, Shahad Alsaiari, and Michael Margineanu for their help in preparing the biological specimens. This work was supported by King Abdullah University of Science and Technology Individual Baseline Funding, as well as Center Partnership Funding.