QUANTITATIVE AND MULTIPLEX DETECTION OF EXTRACELLULAR VESICLE-DERIVED MICRORNA BIOMARKERS VIA ROLLING CIRCLE AMPLIFICATION WITHIN ENCODED HYDROGEL MICROPARTICLES

Dana Al Sulaiman; Nidhi Juthani; Patrick S. Doyle

QUANTITATIVE AND MULTIPLEX DETECTION OF EXTRACELLULAR VESICLE-DERIVED MICRORNA BIOMARKERS VIA ROLLING CIRCLE AMPLIFICATION WITHIN ENCODED HYDROGEL MICROPARTICLES

Dana Al Sulaiman, Nidhi Juthani, Patrick S. Doyle

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This work presents a method for quantitative and multiplex detection of extracellular vesicle-derived microRNA, a promising cancer biomarker, via rolling circle amplification within encoded hydrogel particles. By a one-pot extracellular vesicle lysis and microRNA capture step, we avoid the bias and losses associated with standard RNA extraction. The system offers a large dynamic range (3 orders of magnitude), ease of multiplexing, and a limit of detection down to 2.1 zmol (42 aM), demonstrating its utility in clinical applications based on liquid biopsy tests.

Original language	English (US)
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	697-698
Number of pages	2
ISBN (Print)	9781733419031
State	Published - Jan 1 2021
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2022-09-11

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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Al Sulaiman, D., Juthani, N., & Doyle, P. S. (2021). QUANTITATIVE AND MULTIPLEX DETECTION OF EXTRACELLULAR VESICLE-DERIVED MICRORNA BIOMARKERS VIA ROLLING CIRCLE AMPLIFICATION WITHIN ENCODED HYDROGEL MICROPARTICLES. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 697-698). Chemical and Biological Microsystems Society.

Al Sulaiman, Dana ; Juthani, Nidhi ; Doyle, Patrick S. / QUANTITATIVE AND MULTIPLEX DETECTION OF EXTRACELLULAR VESICLE-DERIVED MICRORNA BIOMARKERS VIA ROLLING CIRCLE AMPLIFICATION WITHIN ENCODED HYDROGEL MICROPARTICLES. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 697-698

@inproceedings{b24ae95c893b4b8083b653949a8918fe,

title = "QUANTITATIVE AND MULTIPLEX DETECTION OF EXTRACELLULAR VESICLE-DERIVED MICRORNA BIOMARKERS VIA ROLLING CIRCLE AMPLIFICATION WITHIN ENCODED HYDROGEL MICROPARTICLES",

abstract = "This work presents a method for quantitative and multiplex detection of extracellular vesicle-derived microRNA, a promising cancer biomarker, via rolling circle amplification within encoded hydrogel particles. By a one-pot extracellular vesicle lysis and microRNA capture step, we avoid the bias and losses associated with standard RNA extraction. The system offers a large dynamic range (3 orders of magnitude), ease of multiplexing, and a limit of detection down to 2.1 zmol (42 aM), demonstrating its utility in clinical applications based on liquid biopsy tests.",

author = "{Al Sulaiman}, Dana and Nidhi Juthani and Doyle, {Patrick S.}",

note = "Generated from Scopus record by KAUST IRTS on 2022-09-11",

year = "2021",

month = jan,

day = "1",

language = "English (US)",

isbn = "9781733419031",

pages = "697--698",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

}

Al Sulaiman, D, Juthani, N & Doyle, PS 2021, QUANTITATIVE AND MULTIPLEX DETECTION OF EXTRACELLULAR VESICLE-DERIVED MICRORNA BIOMARKERS VIA ROLLING CIRCLE AMPLIFICATION WITHIN ENCODED HYDROGEL MICROPARTICLES. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 697-698.

QUANTITATIVE AND MULTIPLEX DETECTION OF EXTRACELLULAR VESICLE-DERIVED MICRORNA BIOMARKERS VIA ROLLING CIRCLE AMPLIFICATION WITHIN ENCODED HYDROGEL MICROPARTICLES. / Al Sulaiman, Dana; Juthani, Nidhi; Doyle, Patrick S.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 697-698.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - QUANTITATIVE AND MULTIPLEX DETECTION OF EXTRACELLULAR VESICLE-DERIVED MICRORNA BIOMARKERS VIA ROLLING CIRCLE AMPLIFICATION WITHIN ENCODED HYDROGEL MICROPARTICLES

AU - Al Sulaiman, Dana

AU - Juthani, Nidhi

AU - Doyle, Patrick S.

N1 - Generated from Scopus record by KAUST IRTS on 2022-09-11

PY - 2021/1/1

Y1 - 2021/1/1

N2 - This work presents a method for quantitative and multiplex detection of extracellular vesicle-derived microRNA, a promising cancer biomarker, via rolling circle amplification within encoded hydrogel particles. By a one-pot extracellular vesicle lysis and microRNA capture step, we avoid the bias and losses associated with standard RNA extraction. The system offers a large dynamic range (3 orders of magnitude), ease of multiplexing, and a limit of detection down to 2.1 zmol (42 aM), demonstrating its utility in clinical applications based on liquid biopsy tests.

AB - This work presents a method for quantitative and multiplex detection of extracellular vesicle-derived microRNA, a promising cancer biomarker, via rolling circle amplification within encoded hydrogel particles. By a one-pot extracellular vesicle lysis and microRNA capture step, we avoid the bias and losses associated with standard RNA extraction. The system offers a large dynamic range (3 orders of magnitude), ease of multiplexing, and a limit of detection down to 2.1 zmol (42 aM), demonstrating its utility in clinical applications based on liquid biopsy tests.

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M3 - Conference contribution

SN - 9781733419031

SP - 697

EP - 698

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

ER -

QUANTITATIVE AND MULTIPLEX DETECTION OF EXTRACELLULAR VESICLE-DERIVED MICRORNA BIOMARKERS VIA ROLLING CIRCLE AMPLIFICATION WITHIN ENCODED HYDROGEL MICROPARTICLES

Abstract

Bibliographical note

UN SDGs

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