Quantification of binary diffusion in protein crystals

Aleksandar Cvetkovic; Cristian Picioreanu; Adrie J.J. Straathof; Rajamani Krishna; Luuk A.M. Van Der Wielen

doi:10.1021/jp050289c

Quantification of binary diffusion in protein crystals

Aleksandar Cvetkovic, Cristian Picioreanu, Adrie J.J. Straathof, Rajamani Krishna, Luuk A.M. Van Der Wielen

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The use of confocal laser scanning microscopy for visualization and quantification of binary diffusion within anisotropic porous material is described here for the first time. The dynamics of adsorption profiles of dianionic fluorescein, zwitterionic rhodamine B, and their mixture in the cationic native orthorhombic lysozyme crystal were subsequently analyzed. All data could be described by a classical pore diffusion model. There was no change in the adsorption characteristics, but diffusion decreased with the introduction of a second solute in the solution. It was found that diffusion is determined by the combination of steric and electrostatic interactions, while adsorption is dependent on electrostatic and hydrophobic interactions. Thus, it was established that the outcome of binary transport depends on the solute, protein, and crystal characteristics. © 2005 American Chemical Society.

Original language	English (US)
Pages (from-to)	10561-10566
Number of pages	6
Journal	Journal of Physical Chemistry B
Volume	109
Issue number	21
DOIs	https://doi.org/10.1021/jp050289c
State	Published - Jun 2 2005
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2022-09-13

ASJC Scopus subject areas

Materials Chemistry
Surfaces, Coatings and Films
Physical and Theoretical Chemistry

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title = "Quantification of binary diffusion in protein crystals",

abstract = "The use of confocal laser scanning microscopy for visualization and quantification of binary diffusion within anisotropic porous material is described here for the first time. The dynamics of adsorption profiles of dianionic fluorescein, zwitterionic rhodamine B, and their mixture in the cationic native orthorhombic lysozyme crystal were subsequently analyzed. All data could be described by a classical pore diffusion model. There was no change in the adsorption characteristics, but diffusion decreased with the introduction of a second solute in the solution. It was found that diffusion is determined by the combination of steric and electrostatic interactions, while adsorption is dependent on electrostatic and hydrophobic interactions. Thus, it was established that the outcome of binary transport depends on the solute, protein, and crystal characteristics. {\textcopyright} 2005 American Chemical Society.",

author = "Aleksandar Cvetkovic and Cristian Picioreanu and Straathof, {Adrie J.J.} and Rajamani Krishna and {Van Der Wielen}, {Luuk A.M.}",

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T1 - Quantification of binary diffusion in protein crystals

AU - Cvetkovic, Aleksandar

AU - Picioreanu, Cristian

AU - Straathof, Adrie J.J.

AU - Krishna, Rajamani

AU - Van Der Wielen, Luuk A.M.

N1 - Generated from Scopus record by KAUST IRTS on 2022-09-13

PY - 2005/6/2

Y1 - 2005/6/2

N2 - The use of confocal laser scanning microscopy for visualization and quantification of binary diffusion within anisotropic porous material is described here for the first time. The dynamics of adsorption profiles of dianionic fluorescein, zwitterionic rhodamine B, and their mixture in the cationic native orthorhombic lysozyme crystal were subsequently analyzed. All data could be described by a classical pore diffusion model. There was no change in the adsorption characteristics, but diffusion decreased with the introduction of a second solute in the solution. It was found that diffusion is determined by the combination of steric and electrostatic interactions, while adsorption is dependent on electrostatic and hydrophobic interactions. Thus, it was established that the outcome of binary transport depends on the solute, protein, and crystal characteristics. © 2005 American Chemical Society.

AB - The use of confocal laser scanning microscopy for visualization and quantification of binary diffusion within anisotropic porous material is described here for the first time. The dynamics of adsorption profiles of dianionic fluorescein, zwitterionic rhodamine B, and their mixture in the cationic native orthorhombic lysozyme crystal were subsequently analyzed. All data could be described by a classical pore diffusion model. There was no change in the adsorption characteristics, but diffusion decreased with the introduction of a second solute in the solution. It was found that diffusion is determined by the combination of steric and electrostatic interactions, while adsorption is dependent on electrostatic and hydrophobic interactions. Thus, it was established that the outcome of binary transport depends on the solute, protein, and crystal characteristics. © 2005 American Chemical Society.

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U2 - 10.1021/jp050289c

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JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

IS - 21

ER -

Quantification of binary diffusion in protein crystals

Abstract

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