Quantification of binary diffusion in protein crystals

Aleksandar Cvetkovic, Cristian Picioreanu, Adrie J.J. Straathof, Rajamani Krishna, Luuk A.M. Van Der Wielen

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The use of confocal laser scanning microscopy for visualization and quantification of binary diffusion within anisotropic porous material is described here for the first time. The dynamics of adsorption profiles of dianionic fluorescein, zwitterionic rhodamine B, and their mixture in the cationic native orthorhombic lysozyme crystal were subsequently analyzed. All data could be described by a classical pore diffusion model. There was no change in the adsorption characteristics, but diffusion decreased with the introduction of a second solute in the solution. It was found that diffusion is determined by the combination of steric and electrostatic interactions, while adsorption is dependent on electrostatic and hydrophobic interactions. Thus, it was established that the outcome of binary transport depends on the solute, protein, and crystal characteristics. © 2005 American Chemical Society.
Original languageEnglish (US)
Pages (from-to)10561-10566
Number of pages6
JournalJournal of Physical Chemistry B
Volume109
Issue number21
DOIs
StatePublished - Jun 2 2005
Externally publishedYes

ASJC Scopus subject areas

  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Physical and Theoretical Chemistry

Fingerprint

Dive into the research topics of 'Quantification of binary diffusion in protein crystals'. Together they form a unique fingerprint.

Cite this