Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media

Veronica Astro*, Gustavo Ramirez-Calderon, Antonio Adamo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) is a powerful tool to investigate early cardiogenesis and model diseases in vitro. Here, we present an optimized protocol to obtain contracting hPSCs-derived cardiomyocytes using a ready-to-use kit. We describe steps for hPSC culture and differentiation to cardiomyocytes including the identification of key parameters such as starting cell confluency and temperature. We then detail immunofluorescence, flow cytometry, and the quantification of cardiomyocytes' calcium spikes using live imaging. For complete details on the use and execution of this protocol, please refer to Astro et al.1

Original languageEnglish (US)
Article number102252
JournalSTAR Protocols
Volume4
Issue number2
DOIs
StatePublished - Jun 16 2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)

Keywords

  • Cell Biology
  • Cell Culture
  • Cell Differentiation
  • Flow Cytometry/Mass Cytometry
  • Microscopy
  • Stem Cells

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology

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