Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media

Veronica Astro; Gustavo Ramirez Calderon; Antonio Adamo

doi:10.1016/j.xpro.2023.102252

Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media

Veronica Astro, Gustavo Ramirez Calderon, Antonio Adamo

Research output: Contribution to journal › Article › peer-review

Abstract

The derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) is a powerful tool to investigate early cardiogenesis and model diseases in vitro. Here, we present an optimized protocol to obtain contracting hPSCs-derived cardiomyocytes using a ready-to-use kit. We describe steps for hPSC culture and differentiation to cardiomyocytes including the identification of key parameters such as starting cell confluency and temperature. We then detail immunofluorescence, flow cytometry, and the quantification of cardiomyocytes' calcium spikes using live imaging. For complete details on the use and execution of this protocol, please refer to Astro et al.1

Original language	English (US)
Pages (from-to)	102252
Journal	STAR Protocols
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2023.102252
State	Published - Apr 14 2023

Bibliographical note

KAUST Repository Item: Exported on 2023-05-10
Acknowledged KAUST grant number(s): URF/1/4012-01-01
Acknowledgements: This work was supported by a competitive research (CRG) grant number URF/1/4012-01-01 from King Abdullah University of Science and Technology to A.A.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2023.102252

https://repository.kaust.edu.sa/bitstream/10754/691592/1/1-s2.0-S2666166723002101-main.pdf

Cite this

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title = "Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media",

abstract = "The derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) is a powerful tool to investigate early cardiogenesis and model diseases in vitro. Here, we present an optimized protocol to obtain contracting hPSCs-derived cardiomyocytes using a ready-to-use kit. We describe steps for hPSC culture and differentiation to cardiomyocytes including the identification of key parameters such as starting cell confluency and temperature. We then detail immunofluorescence, flow cytometry, and the quantification of cardiomyocytes' calcium spikes using live imaging. For complete details on the use and execution of this protocol, please refer to Astro et al.1",

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AU - Astro, Veronica

AU - Ramirez Calderon, Gustavo

AU - Adamo, Antonio

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Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media

Abstract

Bibliographical note

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