Protocol for characterizing strigolactones released by plant roots

Jian You Wang; Guan Ting Erica Chen; Muhammad Jamil; Justine Braguy; Salim Sioud; Kit Xi Liew; Aparna Balakrishna; Salim Al-Babili

doi:10.1016/j.xpro.2022.101352

Protocol for characterizing strigolactones released by plant roots

Jian You Wang^*, Guan Ting Erica Chen, Muhammad Jamil, Justine Braguy, Salim Sioud, Kit Xi Liew, Aparna Balakrishna, Salim Al-Babili^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C₁₈ column for SL extraction, followed by liquid chromatography-mass spectrometry (LC-MS) for SL quantification. We then describe image processing by SeedQuant, an open-source artificial-intelligence-based software, for measuring the biological activity of SLs in inducing root parasitic plant seed germination. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019) and Braguy et al. (2021).

Original language	English (US)
Article number	101352
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101352
State	Published - Jun 17 2022

Bibliographical note

Funding Information:
We thank Dr. Tadao Asami for providing D 6 -5-deoxystrigol standard. This work was supported by the Competitive Research Grants ( CRG2017 and CRG2020 ) and baseline funding from King Abdullah University of Science and Technology , and the Bill & Melinda Gates Foundation (grant number OPP1136424 ) given to S. A.-B..

Publisher Copyright:
© 2022 The Authors

Keywords

Mass Spectrometry
Metabolism
Metabolomics
Model Organisms
Plant sciences

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Immunology and Microbiology
General Medicine

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10.1016/j.xpro.2022.101352

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title = "Protocol for characterizing strigolactones released by plant roots",

abstract = "The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C18 column for SL extraction, followed by liquid chromatography-mass spectrometry (LC-MS) for SL quantification. We then describe image processing by SeedQuant, an open-source artificial-intelligence-based software, for measuring the biological activity of SLs in inducing root parasitic plant seed germination. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019) and Braguy et al. (2021).",

keywords = "Mass Spectrometry, Metabolism, Metabolomics, Model Organisms, Plant sciences",

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note = "Funding Information: We thank Dr. Tadao Asami for providing D 6 -5-deoxystrigol standard. This work was supported by the Competitive Research Grants ( CRG2017 and CRG2020 ) and baseline funding from King Abdullah University of Science and Technology , and the Bill & Melinda Gates Foundation (grant number OPP1136424 ) given to S. A.-B.. Publisher Copyright: {\textcopyright} 2022 The Authors",

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doi = "10.1016/j.xpro.2022.101352",

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AU - Wang, Jian You

AU - Chen, Guan Ting Erica

AU - Jamil, Muhammad

AU - Braguy, Justine

AU - Sioud, Salim

AU - Liew, Kit Xi

AU - Balakrishna, Aparna

AU - Al-Babili, Salim

N1 - Funding Information: We thank Dr. Tadao Asami for providing D 6 -5-deoxystrigol standard. This work was supported by the Competitive Research Grants ( CRG2017 and CRG2020 ) and baseline funding from King Abdullah University of Science and Technology , and the Bill & Melinda Gates Foundation (grant number OPP1136424 ) given to S. A.-B.. Publisher Copyright: © 2022 The Authors

PY - 2022/6/17

Y1 - 2022/6/17

N2 - The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C18 column for SL extraction, followed by liquid chromatography-mass spectrometry (LC-MS) for SL quantification. We then describe image processing by SeedQuant, an open-source artificial-intelligence-based software, for measuring the biological activity of SLs in inducing root parasitic plant seed germination. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019) and Braguy et al. (2021).

AB - The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C18 column for SL extraction, followed by liquid chromatography-mass spectrometry (LC-MS) for SL quantification. We then describe image processing by SeedQuant, an open-source artificial-intelligence-based software, for measuring the biological activity of SLs in inducing root parasitic plant seed germination. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019) and Braguy et al. (2021).

KW - Mass Spectrometry

KW - Metabolism

KW - Metabolomics

KW - Model Organisms

KW - Plant sciences

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ER -

Protocol for characterizing strigolactones released by plant roots

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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