Protocol for characterizing strigolactones released by plant roots

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15 Scopus citations


The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C18 column for SL extraction, followed by liquid chromatography-mass spectrometry (LC-MS) for SL quantification. We then describe image processing by SeedQuant, an open-source artificial-intelligence-based software, for measuring the biological activity of SLs in inducing root parasitic plant seed germination. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019) and Braguy et al. (2021).

Original languageEnglish (US)
Article number101352
JournalSTAR Protocols
Issue number2
StatePublished - Jun 17 2022

Bibliographical note

Funding Information:
We thank Dr. Tadao Asami for providing D 6 -5-deoxystrigol standard. This work was supported by the Competitive Research Grants ( CRG2017 and CRG2020 ) and baseline funding from King Abdullah University of Science and Technology , and the Bill & Melinda Gates Foundation (grant number OPP1136424 ) given to S. A.-B..

Publisher Copyright:
© 2022 The Authors


  • Mass Spectrometry
  • Metabolism
  • Metabolomics
  • Model Organisms
  • Plant sciences

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology
  • General Medicine


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