Vibrio spp. have the ability to form biofilms, which may contribute to the subsequent successful colonization by microfouling and macrofouling organisms. The effects of an antifouling compound, poly-ether B, on Vibrio sp. 010 were investigated using flow cytometry, proteomics, and metabolomics. A 2-D gel-based proteomic analysis was used to identify proteins responsive to poly-ether B treatment. The profiles of biofilm metabolites were analyzed by ultra-performance liquid chromatography-mass spectrometry. Poly-ether B caused a significant reduction in viability. The proteins affected by the treatment were related to nucleotide metabolism, the glyoxylate cycle, and stress responses. Metabolites such as tripeptides, fatty acids, and quorum-sensing molecules were regulated differentially. Down-regulation of proteins and metabolites potentially led to a loss in colonisation ability, thereby affecting the structure of the biofilm. These results suggest that the proteins and metabolites identified may serve as target molecules for potent antifouling compounds. © 2013 Copyright Taylor and Francis Group, LLC.
Bibliographical noteKAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors are grateful to Dr On On Lee, Ms Cherry Kwan, and Ms Emily Giles for proof-reading the manuscript and Ms Lisa Soo, Dr Jin Sun and Ms Crystal Lai for technical help with the experiments. This study was supported by a grant from China Ocean Mineral Resources Research and Development (DY125-15-T-02), a grant from Sanya Institute of Deep-Sea Science and Engineering (SIDSSE-201206), and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology to PY Qian.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Water Science and Technology
- Aquatic Science