Proteomic and metabolomic profiles of marine Vibrio sp. 010 in response to an antifoulant challenge

Kondethimmanahalli Chandramouli, Swagatika Dash, Yu Zhang, Timothy Ravasi, Pei-Yuan Qian

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Vibrio spp. have the ability to form biofilms, which may contribute to the subsequent successful colonization by microfouling and macrofouling organisms. The effects of an antifouling compound, poly-ether B, on Vibrio sp. 010 were investigated using flow cytometry, proteomics, and metabolomics. A 2-D gel-based proteomic analysis was used to identify proteins responsive to poly-ether B treatment. The profiles of biofilm metabolites were analyzed by ultra-performance liquid chromatography-mass spectrometry. Poly-ether B caused a significant reduction in viability. The proteins affected by the treatment were related to nucleotide metabolism, the glyoxylate cycle, and stress responses. Metabolites such as tripeptides, fatty acids, and quorum-sensing molecules were regulated differentially. Down-regulation of proteins and metabolites potentially led to a loss in colonisation ability, thereby affecting the structure of the biofilm. These results suggest that the proteins and metabolites identified may serve as target molecules for potent antifouling compounds. © 2013 Copyright Taylor and Francis Group, LLC.
Original languageEnglish (US)
Pages (from-to)789-802
Number of pages14
JournalBiofouling
Volume29
Issue number7
DOIs
StatePublished - Aug 2013

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors are grateful to Dr On On Lee, Ms Cherry Kwan, and Ms Emily Giles for proof-reading the manuscript and Ms Lisa Soo, Dr Jin Sun and Ms Crystal Lai for technical help with the experiments. This study was supported by a grant from China Ocean Mineral Resources Research and Development (DY125-15-T-02), a grant from Sanya Institute of Deep-Sea Science and Engineering (SIDSSE-201206), and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology to PY Qian.

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Aquatic Science

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