Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16T isolated from Lake Cadagno

Nicola Storelli*, Maged M. Saad, Niels Ulrik Frigaard, Xavier Perret, Mauro Tonolla

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.

Original languageEnglish (US)
Pages (from-to)17-30
Number of pages14
JournalEuPA Open Proteomics
StatePublished - Mar 2014
Externally publishedYes

Bibliographical note

Funding Information:
Financial support for this project was provided by the University of Geneva, the Institute of Microbiology and State of Ticino, and the Swiss National Science Foundation (grant no. 31003A-116591 ). The authors are indebted to Cinzia Benagli, Sophie De Respinis and Natalia Giot for technical and moral support. Furthermore, we are grateful to D. Burri and M. Wittwer from SPIEZ Laboratory, Federal Office for Civil Protection (FOCP), Spiez (Switzlerland), for their technical support. The authors would also like to thank the FEMS and Alpine Biology Center Foundation (ABC) for their support.


  • Dicarboxylate/4-hydroxybutyrate (DC/HB) cycle
  • Granules of poly(3-hydroxybutyrate) (PHB)
  • Phototrophic sulfur bacteria

ASJC Scopus subject areas

  • Biochemistry


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