TY - JOUR
T1 - Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16T isolated from Lake Cadagno
AU - Storelli, Nicola
AU - Saad, Maged
AU - Frigaard, Niels Ulrik
AU - Perret, Xavier
AU - Tonolla, Mauro
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.
AB - Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.
KW - Dicarboxylate/4-hydroxybutyrate (DC/HB) cycle
KW - Granules of poly(3-hydroxybutyrate) (PHB)
KW - Phototrophic sulfur bacteria
UR - http://www.scopus.com/inward/record.url?scp=84895524782&partnerID=8YFLogxK
U2 - 10.1016/j.euprot.2013.11.010
DO - 10.1016/j.euprot.2013.11.010
M3 - Article
AN - SCOPUS:84895524782
VL - 2
SP - 17
EP - 30
JO - EuPA Open Proteomics
JF - EuPA Open Proteomics
SN - 2212-9685
ER -