Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages

Mohammad Zeeshan, Rajan Pandey, Amit Subudhi, David J P Ferguson, Gursimran Kaur, Ravish Rashpa, Raushan Nugmanova, Declan Brady, Andrew R. Bottrill, Sue Vaughan, Mathieu Brochet, Mathieu Bollen, Arnab Pain, Anthony A. Holder, David S. Guttery, Rita Tewari

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


AbstractPP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the malaria parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is implicated in both mitotic exit and, potentially, establishing cell polarity during zygote development in the mosquito midgut, suggesting that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.
Original languageEnglish (US)
JournalCommunications Biology
Issue number1
StatePublished - Jun 18 2021


Dive into the research topics of 'Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages'. Together they form a unique fingerprint.

Cite this