Protein hydrolysis by protease isolated from tuna spleen by membrane filtration: A comparative study with commercial proteases

Zhen Yu Li; Wirote Youravong; Aran H-Kittikun

doi:10.1016/j.lwt.2009.07.002

Protein hydrolysis by protease isolated from tuna spleen by membrane filtration: A comparative study with commercial proteases

Zhen Yu Li, Wirote Youravong, Aran H-Kittikun^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Membrane filtration is considered to be a low-cost and large scale method for recovery and purification of enzymes. The trypsin-like serine protease (TSP) was recovered and purified by microfiltration and ultrafiltration from the extract of the spleen of the yellowfin tuna (Thunnus albacores). The partially purified TSP has the activity of 96.5 U/mL and purity of 74.2 U/mg protein based on casein digesting unit. It has the molecular weight of 24 kDa proved by SDS-PAGE. The potential application of TSP in the protein hydrolysis was investigated in comparison to the use of two commercial proteases, i.e. Alcalase^® 0.6 L and Delvo-Pro under their optimal hydrolysis conditions. The effects of enzymes, substrates and enzyme to substrate ratio on the degree of hydrolysis (DH) were studied. Alcalase showed the highest DH for both casein and soybean isolate. TSP showed higher DH than Delvo-Pro when casein was employed as the substrate. However, TSP showed the lowest DH when soybean protein was used as the substrate. The present study proved that TSP recovered and purified from the tuna canning waste by membrane filtration can be used for protein hydrolysis as well as other commercial proteases.

Original language	English (US)
Pages (from-to)	166-172
Number of pages	7
Journal	LWT
Volume	43
Issue number	1
DOIs	https://doi.org/10.1016/j.lwt.2009.07.002
State	Published - Jan 2010

Bibliographical note

Funding Information:
The authors gratefully acknowledge Faculty of Agro-Industry and Graduate School, Prince of Songkla University for financial support of the work. The authors also express their gratitude to Chotiwat Manufacturing Co. Ltd. for providing yellowfin tuna viscera and Songkhla Canning Public Co Ltd (Thailand) for providing Delvo-Pro used in this study.

Keywords

Membrane filtration
Protease
Protein hydrolysis
Tuna spleen

ASJC Scopus subject areas

Food Science

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10.1016/j.lwt.2009.07.002

Cite this

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title = "Protein hydrolysis by protease isolated from tuna spleen by membrane filtration: A comparative study with commercial proteases",

abstract = "Membrane filtration is considered to be a low-cost and large scale method for recovery and purification of enzymes. The trypsin-like serine protease (TSP) was recovered and purified by microfiltration and ultrafiltration from the extract of the spleen of the yellowfin tuna (Thunnus albacores). The partially purified TSP has the activity of 96.5 U/mL and purity of 74.2 U/mg protein based on casein digesting unit. It has the molecular weight of 24 kDa proved by SDS-PAGE. The potential application of TSP in the protein hydrolysis was investigated in comparison to the use of two commercial proteases, i.e. Alcalase{\textregistered} 0.6 L and Delvo-Pro under their optimal hydrolysis conditions. The effects of enzymes, substrates and enzyme to substrate ratio on the degree of hydrolysis (DH) were studied. Alcalase showed the highest DH for both casein and soybean isolate. TSP showed higher DH than Delvo-Pro when casein was employed as the substrate. However, TSP showed the lowest DH when soybean protein was used as the substrate. The present study proved that TSP recovered and purified from the tuna canning waste by membrane filtration can be used for protein hydrolysis as well as other commercial proteases.",

keywords = "Membrane filtration, Protease, Protein hydrolysis, Tuna spleen",

author = "Li, {Zhen Yu} and Wirote Youravong and Aran H-Kittikun",

note = "Funding Information: The authors gratefully acknowledge Faculty of Agro-Industry and Graduate School, Prince of Songkla University for financial support of the work. The authors also express their gratitude to Chotiwat Manufacturing Co. Ltd. for providing yellowfin tuna viscera and Songkhla Canning Public Co Ltd (Thailand) for providing Delvo-Pro used in this study.",

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AU - Li, Zhen Yu

AU - Youravong, Wirote

AU - H-Kittikun, Aran

N1 - Funding Information: The authors gratefully acknowledge Faculty of Agro-Industry and Graduate School, Prince of Songkla University for financial support of the work. The authors also express their gratitude to Chotiwat Manufacturing Co. Ltd. for providing yellowfin tuna viscera and Songkhla Canning Public Co Ltd (Thailand) for providing Delvo-Pro used in this study.

PY - 2010/1

Y1 - 2010/1

N2 - Membrane filtration is considered to be a low-cost and large scale method for recovery and purification of enzymes. The trypsin-like serine protease (TSP) was recovered and purified by microfiltration and ultrafiltration from the extract of the spleen of the yellowfin tuna (Thunnus albacores). The partially purified TSP has the activity of 96.5 U/mL and purity of 74.2 U/mg protein based on casein digesting unit. It has the molecular weight of 24 kDa proved by SDS-PAGE. The potential application of TSP in the protein hydrolysis was investigated in comparison to the use of two commercial proteases, i.e. Alcalase® 0.6 L and Delvo-Pro under their optimal hydrolysis conditions. The effects of enzymes, substrates and enzyme to substrate ratio on the degree of hydrolysis (DH) were studied. Alcalase showed the highest DH for both casein and soybean isolate. TSP showed higher DH than Delvo-Pro when casein was employed as the substrate. However, TSP showed the lowest DH when soybean protein was used as the substrate. The present study proved that TSP recovered and purified from the tuna canning waste by membrane filtration can be used for protein hydrolysis as well as other commercial proteases.

AB - Membrane filtration is considered to be a low-cost and large scale method for recovery and purification of enzymes. The trypsin-like serine protease (TSP) was recovered and purified by microfiltration and ultrafiltration from the extract of the spleen of the yellowfin tuna (Thunnus albacores). The partially purified TSP has the activity of 96.5 U/mL and purity of 74.2 U/mg protein based on casein digesting unit. It has the molecular weight of 24 kDa proved by SDS-PAGE. The potential application of TSP in the protein hydrolysis was investigated in comparison to the use of two commercial proteases, i.e. Alcalase® 0.6 L and Delvo-Pro under their optimal hydrolysis conditions. The effects of enzymes, substrates and enzyme to substrate ratio on the degree of hydrolysis (DH) were studied. Alcalase showed the highest DH for both casein and soybean isolate. TSP showed higher DH than Delvo-Pro when casein was employed as the substrate. However, TSP showed the lowest DH when soybean protein was used as the substrate. The present study proved that TSP recovered and purified from the tuna canning waste by membrane filtration can be used for protein hydrolysis as well as other commercial proteases.

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Protein hydrolysis by protease isolated from tuna spleen by membrane filtration: A comparative study with commercial proteases

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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