Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov

Camila Gazolla Volpiano; Fernando Hayashi Sant'Anna; Fábio Faria da Mota; Vartul Sangal; Iain Sutcliffe; Madhaiyan Munusamy; Venkatakrishnan Sivaraj Saravanan; Wah-Seng See-Too; Luciane Maria Pereira Passaglia; Alexandre Soares Rosado

doi:10.1016/j.syapm.2021.126223

Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov

Camila Gazolla Volpiano, Fernando Hayashi Sant'Anna, Fábio Faria da Mota, Vartul Sangal, Iain Sutcliffe, Madhaiyan Munusamy, Venkatakrishnan Sivaraj Saravanan, Wah-Seng See-Too, Luciane Maria Pereira Passaglia, Alexandre Soares Rosado

King Abdullah University of Science and Technology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Streptomyces thermoautotrophicus UBT1T has been suggested to merit generic status due to its phylogenetic placement and distinctive phenotypes among Actinomycetia. To evaluate whether ‘S. thermoautotrophicus’ represents a higher taxonomic rank, ‘S. thermoautotrophicus’ strains UBT1T and H1 were compared to Actinomycetia using 16S rRNA gene sequences and comparative genome analyses. The UBT1T and H1 genomes each contain at least two different 16S rRNA sequences, which are closely related to those of Acidothermus cellulolyticus (order Acidothermales). In multigene-based phylogenomic trees, UBT1T and H1 typically formed a sister group to the Streptosporangiales-Acidothermales clade. The Average Amino Acid Identity, Percentage of Conserved Proteins, and whole-genome Average Nucleotide Identity (Alignment Fraction) values were ≤58.5%, ≤48%, ≤75.5% (0.3) between ‘S. thermoautotrophicus’ and Streptosporangiales members, all below the respective thresholds for delineating genera. The values for genomics comparisons between strains UBT1T and H1 with Acidothermales, as well as members of the genus Streptomyces, were even lower. A review of the ‘S. thermoautotrophicus’ proteomic profiles and KEGG orthology demonstrated that UBT1T and H1 present pronounced differences, both tested and predicted, in phenotypic and chemotaxonomic characteristics compared to its sister clades and Streptomyces. The distinct phylogenetic position and the combination of genotypic and phenotypic characteristics justify the proposal of Carbonactinospora gen. nov., with the type species Carbonactinospora thermoautotrophica comb. nov. (type strain UBT1T, = DSM 100163T = KCTC 49540T) belonging to Carbonactinosporaceae fam. nov. within Actinomycetia.

Original language	English (US)
Pages (from-to)	126223
Journal	Systematic and Applied Microbiology
DOIs	https://doi.org/10.1016/j.syapm.2021.126223
State	Published - Jun 10 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-06-16
Acknowledged KAUST grant number(s): BAS/1/1096-01-01
Acknowledgements: This work was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil), the CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil) and KAUST (BAS/1/1096-01-01).

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Ecology, Evolution, Behavior and Systematics
Microbiology

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10.1016/j.syapm.2021.126223

https://repository.kaust.edu.sa/bitstream/10754/669586/1/Proposal%20of_1-s2.0-S0723202021000461-main%20%281%29.pdf

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Volpiano, C. G., Sant'Anna, F. H., da Mota, F. F., Sangal, V., Sutcliffe, I., Munusamy, M., Saravanan, V. S., See-Too, W.-S., Passaglia, L. M. P., & Rosado, A. S. (2021). Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov. Systematic and Applied Microbiology, 126223. https://doi.org/10.1016/j.syapm.2021.126223

@article{cde6ca5de7d046f4a4aec604a5469ceb,

title = "Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov",

abstract = "Streptomyces thermoautotrophicus UBT1T has been suggested to merit generic status due to its phylogenetic placement and distinctive phenotypes among Actinomycetia. To evaluate whether {\textquoteleft}S. thermoautotrophicus{\textquoteright} represents a higher taxonomic rank, {\textquoteleft}S. thermoautotrophicus{\textquoteright} strains UBT1T and H1 were compared to Actinomycetia using 16S rRNA gene sequences and comparative genome analyses. The UBT1T and H1 genomes each contain at least two different 16S rRNA sequences, which are closely related to those of Acidothermus cellulolyticus (order Acidothermales). In multigene-based phylogenomic trees, UBT1T and H1 typically formed a sister group to the Streptosporangiales-Acidothermales clade. The Average Amino Acid Identity, Percentage of Conserved Proteins, and whole-genome Average Nucleotide Identity (Alignment Fraction) values were ≤58.5%, ≤48%, ≤75.5% (0.3) between {\textquoteleft}S. thermoautotrophicus{\textquoteright} and Streptosporangiales members, all below the respective thresholds for delineating genera. The values for genomics comparisons between strains UBT1T and H1 with Acidothermales, as well as members of the genus Streptomyces, were even lower. A review of the {\textquoteleft}S. thermoautotrophicus{\textquoteright} proteomic profiles and KEGG orthology demonstrated that UBT1T and H1 present pronounced differences, both tested and predicted, in phenotypic and chemotaxonomic characteristics compared to its sister clades and Streptomyces. The distinct phylogenetic position and the combination of genotypic and phenotypic characteristics justify the proposal of Carbonactinospora gen. nov., with the type species Carbonactinospora thermoautotrophica comb. nov. (type strain UBT1T, = DSM 100163T = KCTC 49540T) belonging to Carbonactinosporaceae fam. nov. within Actinomycetia.",

author = "Volpiano, {Camila Gazolla} and Sant'Anna, {Fernando Hayashi} and {da Mota}, {F{\'a}bio Faria} and Vartul Sangal and Iain Sutcliffe and Madhaiyan Munusamy and Saravanan, {Venkatakrishnan Sivaraj} and Wah-Seng See-Too and Passaglia, {Luciane Maria Pereira} and Rosado, {Alexandre Soares}",

note = "KAUST Repository Item: Exported on 2021-06-16 Acknowledged KAUST grant number(s): BAS/1/1096-01-01 Acknowledgements: This work was supported by CAPES (Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior, Brazil), the CNPq (Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico, Brazil) and KAUST (BAS/1/1096-01-01).",

year = "2021",

month = jun,

day = "10",

doi = "10.1016/j.syapm.2021.126223",

language = "English (US)",

pages = "126223",

journal = "Systematic and Applied Microbiology",

issn = "0723-2020",

publisher = "Elsevier",

}

Volpiano, CG, Sant'Anna, FH, da Mota, FF, Sangal, V, Sutcliffe, I, Munusamy, M, Saravanan, VS, See-Too, W-S, Passaglia, LMP & Rosado, AS 2021, 'Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov', Systematic and Applied Microbiology, pp. 126223. https://doi.org/10.1016/j.syapm.2021.126223

Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov. / Volpiano, Camila Gazolla; Sant'Anna, Fernando Hayashi; da Mota, Fábio Faria et al.
In: Systematic and Applied Microbiology, 10.06.2021, p. 126223.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov

AU - Volpiano, Camila Gazolla

AU - Sant'Anna, Fernando Hayashi

AU - da Mota, Fábio Faria

AU - Sangal, Vartul

AU - Sutcliffe, Iain

AU - Munusamy, Madhaiyan

AU - Saravanan, Venkatakrishnan Sivaraj

AU - See-Too, Wah-Seng

AU - Passaglia, Luciane Maria Pereira

AU - Rosado, Alexandre Soares

N1 - KAUST Repository Item: Exported on 2021-06-16 Acknowledged KAUST grant number(s): BAS/1/1096-01-01 Acknowledgements: This work was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil), the CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil) and KAUST (BAS/1/1096-01-01).

PY - 2021/6/10

Y1 - 2021/6/10

N2 - Streptomyces thermoautotrophicus UBT1T has been suggested to merit generic status due to its phylogenetic placement and distinctive phenotypes among Actinomycetia. To evaluate whether ‘S. thermoautotrophicus’ represents a higher taxonomic rank, ‘S. thermoautotrophicus’ strains UBT1T and H1 were compared to Actinomycetia using 16S rRNA gene sequences and comparative genome analyses. The UBT1T and H1 genomes each contain at least two different 16S rRNA sequences, which are closely related to those of Acidothermus cellulolyticus (order Acidothermales). In multigene-based phylogenomic trees, UBT1T and H1 typically formed a sister group to the Streptosporangiales-Acidothermales clade. The Average Amino Acid Identity, Percentage of Conserved Proteins, and whole-genome Average Nucleotide Identity (Alignment Fraction) values were ≤58.5%, ≤48%, ≤75.5% (0.3) between ‘S. thermoautotrophicus’ and Streptosporangiales members, all below the respective thresholds for delineating genera. The values for genomics comparisons between strains UBT1T and H1 with Acidothermales, as well as members of the genus Streptomyces, were even lower. A review of the ‘S. thermoautotrophicus’ proteomic profiles and KEGG orthology demonstrated that UBT1T and H1 present pronounced differences, both tested and predicted, in phenotypic and chemotaxonomic characteristics compared to its sister clades and Streptomyces. The distinct phylogenetic position and the combination of genotypic and phenotypic characteristics justify the proposal of Carbonactinospora gen. nov., with the type species Carbonactinospora thermoautotrophica comb. nov. (type strain UBT1T, = DSM 100163T = KCTC 49540T) belonging to Carbonactinosporaceae fam. nov. within Actinomycetia.

AB - Streptomyces thermoautotrophicus UBT1T has been suggested to merit generic status due to its phylogenetic placement and distinctive phenotypes among Actinomycetia. To evaluate whether ‘S. thermoautotrophicus’ represents a higher taxonomic rank, ‘S. thermoautotrophicus’ strains UBT1T and H1 were compared to Actinomycetia using 16S rRNA gene sequences and comparative genome analyses. The UBT1T and H1 genomes each contain at least two different 16S rRNA sequences, which are closely related to those of Acidothermus cellulolyticus (order Acidothermales). In multigene-based phylogenomic trees, UBT1T and H1 typically formed a sister group to the Streptosporangiales-Acidothermales clade. The Average Amino Acid Identity, Percentage of Conserved Proteins, and whole-genome Average Nucleotide Identity (Alignment Fraction) values were ≤58.5%, ≤48%, ≤75.5% (0.3) between ‘S. thermoautotrophicus’ and Streptosporangiales members, all below the respective thresholds for delineating genera. The values for genomics comparisons between strains UBT1T and H1 with Acidothermales, as well as members of the genus Streptomyces, were even lower. A review of the ‘S. thermoautotrophicus’ proteomic profiles and KEGG orthology demonstrated that UBT1T and H1 present pronounced differences, both tested and predicted, in phenotypic and chemotaxonomic characteristics compared to its sister clades and Streptomyces. The distinct phylogenetic position and the combination of genotypic and phenotypic characteristics justify the proposal of Carbonactinospora gen. nov., with the type species Carbonactinospora thermoautotrophica comb. nov. (type strain UBT1T, = DSM 100163T = KCTC 49540T) belonging to Carbonactinosporaceae fam. nov. within Actinomycetia.

UR - http://hdl.handle.net/10754/669586

UR - https://linkinghub.elsevier.com/retrieve/pii/S0723202021000461

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DO - 10.1016/j.syapm.2021.126223

M3 - Article

C2 - 34157595

SN - 0723-2020

SP - 126223

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

ER -

Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov

Abstract

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ASJC Scopus subject areas

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