TY - GEN
T1 - Progress and perspectives in digital holographic microscopy applied to life sciences
AU - Marquet, Pierre
AU - Kühn, Jonas
AU - Boss, Daniel
AU - Jourdain, Pascal
AU - Magistretti, Pierre
AU - Pavillon, Nicoals
AU - Depeursinge, Christian
PY - 2010
Y1 - 2010
N2 - Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. The quantitative phase images allow the derivation of highly relevant cell parameters, including dry mass density and spatial distribution. Thanks to a decoupling procedure, cell thickness and intracellular refractive index can be measured separately. Consequently, cell morphology, shape as well as cell membrane fluctuations can be accurately monitor. As far as red blood cell are considered, Mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), two highly relevant clinical parameters, have been measured non-invasively at a single cell level. The DHM nanometric axial and microsecond temporal sensitivities have permitted to measure the red blood cell membrane fluctuations (CMF) over the whole cell surface. In addition, the development of live-cell multimodality microscope combining fluorescence with digital holographic microscopy is presented. The biophysical cell parameters derived from the quantitative phase information in combination with the numerous different specific fluorescent cellular probes allow to this multimodality microscope to address various important issues in cell biology.
AB - Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. The quantitative phase images allow the derivation of highly relevant cell parameters, including dry mass density and spatial distribution. Thanks to a decoupling procedure, cell thickness and intracellular refractive index can be measured separately. Consequently, cell morphology, shape as well as cell membrane fluctuations can be accurately monitor. As far as red blood cell are considered, Mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), two highly relevant clinical parameters, have been measured non-invasively at a single cell level. The DHM nanometric axial and microsecond temporal sensitivities have permitted to measure the red blood cell membrane fluctuations (CMF) over the whole cell surface. In addition, the development of live-cell multimodality microscope combining fluorescence with digital holographic microscopy is presented. The biophysical cell parameters derived from the quantitative phase information in combination with the numerous different specific fluorescent cellular probes allow to this multimodality microscope to address various important issues in cell biology.
UR - http://www.scopus.com/inward/record.url?scp=78149429472&partnerID=8YFLogxK
U2 - 10.1109/WIO.2010.5582513
DO - 10.1109/WIO.2010.5582513
M3 - Conference contribution
AN - SCOPUS:78149429472
SN - 9781424482276
T3 - 2010 9th Euro-American Workshop on Information Optics, WIO 2010
BT - 2010 9th Euro-American Workshop on Information Optics, WIO 2010
T2 - 2010 9th Euro-American Workshop on Information Optics, WIO 2010
Y2 - 12 July 2010 through 16 July 2010
ER -