Profiling of Root Canal Bacterial Communities Associated with Chronic Apical Periodontitis from Brazilian and Norwegian Subjects

José F. Siqueira; Isabela N. Rôças; Gilberto J. Debelian; Flávia L. Carmo; Simone S.M. Paiva; Flávio R.F. Alves; Alexandre S. Rosado

doi:10.1016/j.joen.2008.08.037

Profiling of Root Canal Bacterial Communities Associated with Chronic Apical Periodontitis from Brazilian and Norwegian Subjects

José F. Siqueira, Isabela N. Rôças, Gilberto J. Debelian, Flávia L. Carmo, Simone S.M. Paiva, Flávio R.F. Alves, Alexandre S. Rosado

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37 Scopus citations

Abstract

The aim of this study was to compare the bacterial community profiles of the root canal microbiota associated with chronic apical periodontitis from Brazilian and Norwegian patients using the denaturing gradient gel electrophoresis (DGGE) and the ribosomal intergenic spacer analysis (RISA) approaches. DNA extracted from root canal samples was subjected to polymerase chain reaction using primers appropriate for further DGGE or RISA analysis. The resulting banding patterns representative of the bacterial community structures in samples from the two locations were compared. DGGE and RISA fingerprints showed a great interindividual variability in the bacterial community profiles, irrespective of the geographic location of the patient. However, similarities among the bacterial community DGGE profiles revealed the existence of a geography-related pattern. © 2008 American Association of Endodontists.

Original language	English (US)
Pages (from-to)	1457-1461
Number of pages	5
Journal	Journal of Endodontics
Volume	34
Issue number	12
DOIs	https://doi.org/10.1016/j.joen.2008.08.037
State	Published - Dec 1 2008
Externally published	Yes

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title = "Profiling of Root Canal Bacterial Communities Associated with Chronic Apical Periodontitis from Brazilian and Norwegian Subjects",

abstract = "The aim of this study was to compare the bacterial community profiles of the root canal microbiota associated with chronic apical periodontitis from Brazilian and Norwegian patients using the denaturing gradient gel electrophoresis (DGGE) and the ribosomal intergenic spacer analysis (RISA) approaches. DNA extracted from root canal samples was subjected to polymerase chain reaction using primers appropriate for further DGGE or RISA analysis. The resulting banding patterns representative of the bacterial community structures in samples from the two locations were compared. DGGE and RISA fingerprints showed a great interindividual variability in the bacterial community profiles, irrespective of the geographic location of the patient. However, similarities among the bacterial community DGGE profiles revealed the existence of a geography-related pattern. {\textcopyright} 2008 American Association of Endodontists.",

author = "Siqueira, {Jos{\'e} F.} and R{\^o}{\c c}as, {Isabela N.} and Debelian, {Gilberto J.} and Carmo, {Fl{\'a}via L.} and Paiva, {Simone S.M.} and Alves, {Fl{\'a}vio R.F.} and Rosado, {Alexandre S.}",

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doi = "10.1016/j.joen.2008.08.037",

language = "English (US)",

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T1 - Profiling of Root Canal Bacterial Communities Associated with Chronic Apical Periodontitis from Brazilian and Norwegian Subjects

AU - Siqueira, José F.

AU - Rôças, Isabela N.

AU - Debelian, Gilberto J.

AU - Carmo, Flávia L.

AU - Paiva, Simone S.M.

AU - Alves, Flávio R.F.

AU - Rosado, Alexandre S.

N1 - Generated from Scopus record by KAUST IRTS on 2021-02-16

PY - 2008/12/1

Y1 - 2008/12/1

N2 - The aim of this study was to compare the bacterial community profiles of the root canal microbiota associated with chronic apical periodontitis from Brazilian and Norwegian patients using the denaturing gradient gel electrophoresis (DGGE) and the ribosomal intergenic spacer analysis (RISA) approaches. DNA extracted from root canal samples was subjected to polymerase chain reaction using primers appropriate for further DGGE or RISA analysis. The resulting banding patterns representative of the bacterial community structures in samples from the two locations were compared. DGGE and RISA fingerprints showed a great interindividual variability in the bacterial community profiles, irrespective of the geographic location of the patient. However, similarities among the bacterial community DGGE profiles revealed the existence of a geography-related pattern. © 2008 American Association of Endodontists.

AB - The aim of this study was to compare the bacterial community profiles of the root canal microbiota associated with chronic apical periodontitis from Brazilian and Norwegian patients using the denaturing gradient gel electrophoresis (DGGE) and the ribosomal intergenic spacer analysis (RISA) approaches. DNA extracted from root canal samples was subjected to polymerase chain reaction using primers appropriate for further DGGE or RISA analysis. The resulting banding patterns representative of the bacterial community structures in samples from the two locations were compared. DGGE and RISA fingerprints showed a great interindividual variability in the bacterial community profiles, irrespective of the geographic location of the patient. However, similarities among the bacterial community DGGE profiles revealed the existence of a geography-related pattern. © 2008 American Association of Endodontists.

UR - https://linkinghub.elsevier.com/retrieve/pii/S0099239908007978

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DO - 10.1016/j.joen.2008.08.037

M3 - Article

SN - 0099-2399

VL - 34

SP - 1457

EP - 1461

JO - Journal of Endodontics

JF - Journal of Endodontics

IS - 12

ER -

Profiling of Root Canal Bacterial Communities Associated with Chronic Apical Periodontitis from Brazilian and Norwegian Subjects

Abstract

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