Processing of the hemagglutinin of influenza virus expressed in insect cells by a baculovirus vector

Kazumichi Kuroda; Charlotte Hauser; Rudolf Rott; Walter Doerfler; Hans Dieter Klenk

doi:10.1201/9781351073844

Processing of the hemagglutinin of influenza virus expressed in insect cells by a baculovirus vector

Kazumichi Kuroda, Charlotte Hauser, Rudolf Rott, Walter Doerfler, Hans Dieter Klenk

Bioengineering

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Like other integral membrane proteins, the hemagglutinin (HA) as the major glycoprotein of the influenza virus envelope is translated at the rough endoplasmic reticulum and transported from there through the Golgi apparatus to the plasma membrane. In the course of transport, the HA undergoes posttranslational modifications, including removal of the signal peptide, attachment of N-glycosidic oligosaccharide side chains, acylation, and posttranslational proteolytic cleavage of the precursor HA into the fragments HA1 and HA2, which is necessary for the realization of the fusion activity.

Original language	English (US)
Title of host publication	Invertebrate Cell System Applications
Subtitle of host publication	Volume I
Publisher	CRC Press
Pages	221-230
Number of pages	10
ISBN (Electronic)	9781351082297
ISBN (Print)	0849343739, 9781315894744
DOIs	https://doi.org/10.1201/9781351073844
State	Published - Jan 1 2018

Bibliographical note

Publisher Copyright:
© 1989 by CRC Press, Inc.

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1201/9781351073844

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AU - Klenk, Hans Dieter

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N2 - Like other integral membrane proteins, the hemagglutinin (HA) as the major glycoprotein of the influenza virus envelope is translated at the rough endoplasmic reticulum and transported from there through the Golgi apparatus to the plasma membrane. In the course of transport, the HA undergoes posttranslational modifications, including removal of the signal peptide, attachment of N-glycosidic oligosaccharide side chains, acylation, and posttranslational proteolytic cleavage of the precursor HA into the fragments HA1 and HA2, which is necessary for the realization of the fusion activity.

AB - Like other integral membrane proteins, the hemagglutinin (HA) as the major glycoprotein of the influenza virus envelope is translated at the rough endoplasmic reticulum and transported from there through the Golgi apparatus to the plasma membrane. In the course of transport, the HA undergoes posttranslational modifications, including removal of the signal peptide, attachment of N-glycosidic oligosaccharide side chains, acylation, and posttranslational proteolytic cleavage of the precursor HA into the fragments HA1 and HA2, which is necessary for the realization of the fusion activity.

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Processing of the hemagglutinin of influenza virus expressed in insect cells by a baculovirus vector

Abstract

Bibliographical note

ASJC Scopus subject areas

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