Preparation of hybrid triple-stimuli responsive nanogels based on poly(L-histidine)

Panayiotis Bilalis*, Spyridon Varlas, Aikaterini Kiafa, Athanassios Velentzas, Dimitrios Stravopodis, Hermis Iatrou

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


A series of novel multi-responsive disulfide cross-linked polypeptide nanogels has been synthesized by a one-step ring-opening polymerization process. The pH-responsive core of the prepared nanogels was based on poly(L-histidine), the difunctional N-carboxy anhydride of l-cystine (l-Cys-NCA) was used as a reduction-cleavable cross-linking agent, while the outer hydrophilic corona was comprised of a poly(ethylene oxide) block. Extensive molecular characterization studies were conducted in order to confirm the formation of the desired polymeric nanostructures and also to prove their responsiveness to external stimuli within the physiological values of healthy and cancer tissues. Furthermore, the disruption of the disulfide-bond linkages between the polymeric chains was achieved by the presence of the reductive tripeptide glutathione (GSH), leading to size variations that were monitored by dynamic light scattering (DLS) and size-exclusion chromatography (SEC). "Stealth" properties of the formed nanostructures were examined by zeta potential measurements. The described nanogels are clearly promising candidates for drug delivery applications.

Original languageEnglish (US)
Pages (from-to)1278-1288
Number of pages11
JournalJournal of Polymer Science, Part A: Polymer Chemistry
Issue number9
StatePublished - May 1 2016

Bibliographical note

Publisher Copyright:
© 2015 Wiley Periodicals, Inc.


  • Nanoparticles
  • Poly(L-histidine)
  • Polypeptides
  • Redox chemistry
  • Ring-opening polymerization

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry


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