Predicted expansion of the claudin multigene family

Katsuhiko Mineta; Yasuko Yamamoto; Yuji Yamazaki; Hiroo Tanaka; Yukiyo Tada; Kuniaki Saito; Atsushi Tamura; Michihiro Igarashi; Toshinori Endo; Kosei Takeuchi; Sachiko Tsukita

doi:10.1016/j.febslet.2011.01.028

Predicted expansion of the claudin multigene family

Katsuhiko Mineta, Yasuko Yamamoto, Yuji Yamazaki, Hiroo Tanaka, Yukiyo Tada, Kuniaki Saito, Atsushi Tamura, Michihiro Igarashi, Toshinori Endo, Kosei Takeuchi, Sachiko Tsukita^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

438 Scopus citations

Abstract

Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members. Structured summary: Claudin-25 and ZO-1 colocalize by fluorescence microscopy (View interaction) ZO-1 and Claudin-26 colocalize by fluorescence microscopy (View interaction)

Original language	English (US)
Pages (from-to)	606-612
Number of pages	7
Journal	FEBS Letters
Volume	585
Issue number	4
DOIs	https://doi.org/10.1016/j.febslet.2011.01.028
State	Published - Feb 18 2011
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported in part by a Grant-in-Aid for Creative Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), to Sachiko Tsukita, and by a Grants-in-Aid for Scientific Research from MEXT and for Research and Development Program for New Bio-industry Initiatives, to Kosei Takeuchi.

Keywords

Cell adhesion
Claudin
Epithelial cells
PSI-BLAST
Paracellular barrier
Tight junction

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2011.01.028

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title = "Predicted expansion of the claudin multigene family",

abstract = "Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members. Structured summary: Claudin-25 and ZO-1 colocalize by fluorescence microscopy (View interaction) ZO-1 and Claudin-26 colocalize by fluorescence microscopy (View interaction)",

keywords = "Cell adhesion, Claudin, Epithelial cells, PSI-BLAST, Paracellular barrier, Tight junction",

author = "Katsuhiko Mineta and Yasuko Yamamoto and Yuji Yamazaki and Hiroo Tanaka and Yukiyo Tada and Kuniaki Saito and Atsushi Tamura and Michihiro Igarashi and Toshinori Endo and Kosei Takeuchi and Sachiko Tsukita",

note = "Funding Information: This study was supported in part by a Grant-in-Aid for Creative Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), to Sachiko Tsukita, and by a Grants-in-Aid for Scientific Research from MEXT and for Research and Development Program for New Bio-industry Initiatives, to Kosei Takeuchi. ",

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AU - Mineta, Katsuhiko

AU - Yamamoto, Yasuko

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AU - Tanaka, Hiroo

AU - Tada, Yukiyo

AU - Saito, Kuniaki

AU - Tamura, Atsushi

AU - Igarashi, Michihiro

AU - Endo, Toshinori

AU - Takeuchi, Kosei

AU - Tsukita, Sachiko

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for Creative Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), to Sachiko Tsukita, and by a Grants-in-Aid for Scientific Research from MEXT and for Research and Development Program for New Bio-industry Initiatives, to Kosei Takeuchi.

PY - 2011/2/18

Y1 - 2011/2/18

N2 - Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members. Structured summary: Claudin-25 and ZO-1 colocalize by fluorescence microscopy (View interaction) ZO-1 and Claudin-26 colocalize by fluorescence microscopy (View interaction)

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Predicted expansion of the claudin multigene family

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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