Pre-mRNA splicing repression triggers abiotic stress signaling in plants

Yu Ling; Sahar Alshareef; Haroon Butt; Jorge Lozano-Juste; Lixin Li; Aya A. Galal; Ahmed Moustafa; Afaque Ahmad Imtiyaz Momin; Manal Tashkandi; Dale N. Richardson; Hiroaki Fujii; Stefan T. Arold; Pedro L. Rodriguez; Paula Duque; Magdy M. Mahfouz

doi:10.1111/tpj.13383

Pre-mRNA splicing repression triggers abiotic stress signaling in plants

Yu Ling, Sahar Alshareef, Haroon Butt, Jorge Lozano-Juste, Lixin Li, Aya A. Galal, Ahmed Moustafa, Afaque Ahmad Imtiyaz Momin, Manal Tashkandi, Dale N. Richardson, Hiroaki Fujii, Stefan T. Arold, Pedro L. Rodriguez, Paula Duque, Magdy M. Mahfouz

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Alternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A

Original language	English (US)
Pages (from-to)	291-309
Number of pages	19
Journal	The Plant Journal
Volume	89
Issue number	2
DOIs	https://doi.org/10.1111/tpj.13383
State	Published - Jan 17 2017

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Moussa Benhamed for helpful discussions and suggestions and for providing key materials. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAKPKKK18-uidA. This study was supported by King Abdullah University of Science and Technology. Work in PR's laboratory was funded by grant BIO2014-52537-R from MINECO. Work in PD's laboratory is funded by grant PTDC/BIA-PLA/1084/2014 from FCT. The authors declare no conflicts of interest.

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title = "Pre-mRNA splicing repression triggers abiotic stress signaling in plants",

abstract = "Alternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A",

author = "Yu Ling and Sahar Alshareef and Haroon Butt and Jorge Lozano-Juste and Lixin Li and Galal, {Aya A.} and Ahmed Moustafa and Momin, {Afaque Ahmad Imtiyaz} and Manal Tashkandi and Richardson, {Dale N.} and Hiroaki Fujii and Arold, {Stefan T.} and Rodriguez, {Pedro L.} and Paula Duque and Mahfouz, {Magdy M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Moussa Benhamed for helpful discussions and suggestions and for providing key materials. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAKPKKK18-uidA. This study was supported by King Abdullah University of Science and Technology. Work in PR's laboratory was funded by grant BIO2014-52537-R from MINECO. Work in PD's laboratory is funded by grant PTDC/BIA-PLA/1084/2014 from FCT. The authors declare no conflicts of interest.",

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AU - Alshareef, Sahar

AU - Butt, Haroon

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AU - Galal, Aya A.

AU - Moustafa, Ahmed

AU - Momin, Afaque Ahmad Imtiyaz

AU - Tashkandi, Manal

AU - Richardson, Dale N.

AU - Fujii, Hiroaki

AU - Arold, Stefan T.

AU - Rodriguez, Pedro L.

AU - Duque, Paula

AU - Mahfouz, Magdy M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Moussa Benhamed for helpful discussions and suggestions and for providing key materials. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAKPKKK18-uidA. This study was supported by King Abdullah University of Science and Technology. Work in PR's laboratory was funded by grant BIO2014-52537-R from MINECO. Work in PD's laboratory is funded by grant PTDC/BIA-PLA/1084/2014 from FCT. The authors declare no conflicts of interest.

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N2 - Alternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A

AB - Alternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A

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Pre-mRNA splicing repression triggers abiotic stress signaling in plants

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