TY - JOUR
T1 - Poly(ε-Caprolactone)-Poly(Ethylene Glycol) Tri-Block Copolymer as Quercetin Delivery System for Human Colorectal Carcinoma Cells: Synthesis, Characterization and In Vitro Study
AU - Ferrentino, Nancy
AU - Romano, Maria Preziosa
AU - Zappavigna, Silvia
AU - Abate, Marianna
AU - Del Vecchio, Vitale
AU - Romano, Dario
AU - Germinario, Chiara
AU - Grifa, Celestino
AU - Filosa, Rosanna
AU - Pappalardo, Daniela
N1 - KAUST Repository Item: Exported on 2023-03-02
Acknowledgements: The authors gratefully acknowledged the FAR Università del Sannio and the PON “Ricerca e Innovazione” 2014–2020, azione 4, dottorati su tematiche dell’innovazione.
PY - 2023/2/26
Y1 - 2023/2/26
N2 - Quercetin is a hydrophobic molecule with short blood circulation times and instability. The development of a nano-delivery system formulation of quercetin may increase its bioavailability, resulting in greater tumor suppressing effects. Triblock ABA type polycaprolactone-polyethylenglycol- polycaprolactone (PCL-PEG-PCL) copolymers have been synthetized using ring-opening polymerization of caprolactone from PEG diol. The copolymers were characterized by nuclear magnetic resonance (NMR), diffusion-ordered NMR spectroscopy (DOSY), and gel permeation chromatography (GPC). The triblock copolymers self-assembled in water forming micelles consisting of a core of biodegradable polycaprolactone (PCL) and a corona of polyethylenglycol (PEG). The core-shell PCL-PEG-PCL nanoparticles were able to incorporate quercetin into the core. They were characterized by dynamic light scattering (DLS) and NMR. The cellular uptake efficiency of human colorectal carcinoma cells was quantitatively determined by flow cytometry using nanoparticles loaded with Nile Red as hydrophobic model drug. The cytotoxic effect of quercetin-loaded nanoparticles was evaluated on HCT 116 cells, showing promising results.
AB - Quercetin is a hydrophobic molecule with short blood circulation times and instability. The development of a nano-delivery system formulation of quercetin may increase its bioavailability, resulting in greater tumor suppressing effects. Triblock ABA type polycaprolactone-polyethylenglycol- polycaprolactone (PCL-PEG-PCL) copolymers have been synthetized using ring-opening polymerization of caprolactone from PEG diol. The copolymers were characterized by nuclear magnetic resonance (NMR), diffusion-ordered NMR spectroscopy (DOSY), and gel permeation chromatography (GPC). The triblock copolymers self-assembled in water forming micelles consisting of a core of biodegradable polycaprolactone (PCL) and a corona of polyethylenglycol (PEG). The core-shell PCL-PEG-PCL nanoparticles were able to incorporate quercetin into the core. They were characterized by dynamic light scattering (DLS) and NMR. The cellular uptake efficiency of human colorectal carcinoma cells was quantitatively determined by flow cytometry using nanoparticles loaded with Nile Red as hydrophobic model drug. The cytotoxic effect of quercetin-loaded nanoparticles was evaluated on HCT 116 cells, showing promising results.
UR - http://hdl.handle.net/10754/689836
UR - https://www.mdpi.com/2073-4360/15/5/1179
U2 - 10.3390/polym15051179
DO - 10.3390/polym15051179
M3 - Article
C2 - 36904421
SN - 2073-4360
VL - 15
SP - 1179
JO - Polymers
JF - Polymers
IS - 5
ER -