PLETHORA-WOX5 interaction and subnuclear localization control Arabidopsis root stem cell maintenance

Rebecca C Burkart; Vivien I Strotmann; Gwendolyn Kristin Kirschner; Abdullah Akinci; Laura Czempik; Anika Dolata; Alexis Maizel; Stefanie Weidtkamp-Peters; Yvonne Stahl

doi:10.15252/embr.202154105

PLETHORA-WOX5 interaction and subnuclear localization control Arabidopsis root stem cell maintenance

Rebecca C Burkart, Vivien I Strotmann, Gwendolyn Kristin Kirschner, Abdullah Akinci, Laura Czempik, Anika Dolata, Alexis Maizel, Stefanie Weidtkamp-Peters, Yvonne Stahl

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Maintenance and homeostasis of the stem cell niche (SCN) in the Arabidopsis root is essential for growth and development of all root cell types. The SCN is organized around a quiescent center (QC) maintaining the stemness of cells in direct contact. The key transcription factors (TFs) WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and PLETHORAs (PLTs) are expressed in the SCN where they maintain the QC and regulate distal columella stem cell (CSC) fate. Here, we describe the concerted mutual regulation of the key TFs WOX5 and PLTs on a transcriptional and protein interaction level. Additionally, by applying a novel SCN staining method, we demonstrate that both WOX5 and PLTs regulate root SCN homeostasis as they control QC quiescence and CSC fate interdependently. Moreover, we uncover that some PLTs, especially PLT3, contain intrinsically disordered prion-like domains (PrDs) that are necessary for complex formation with WOX5 and its recruitment to subnuclear microdomains/nuclear bodies (NBs) in the CSCs. We propose that this partitioning of PLT-WOX5 complexes to NBs, possibly by phase separation, is important for CSC fate determination.

Original language	English (US)
Journal	EMBO Reports
DOIs	https://doi.org/10.15252/embr.202154105
State	Published - Apr 4 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-04-15
Acknowledgements: We would like to acknowledge funding of R.C.B. by the Deutsche Forschungsgemeinschaft (DFG) through grant Sta1212/1-1 to Y.S. We thank Andrea Bleckmann for sharing the Greengate Cerulean construct, Renze Heidstra, and Ben Scheres for sharing published lines (PLT

ASJC Scopus subject areas

Biochemistry
Genetics
Molecular Biology

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10.15252/embr.202154105

https://repository.kaust.edu.sa/bitstream/10754/676255/1/EMBO%20Reports%20-%202022%20-%20Burkart%20-%20PLETHORA%e2%80%90WOX5%20interaction%20and%20subnuclear%20localization%20control%20Arabidopsis%20root%20stem%20cell.pdf

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title = "PLETHORA-WOX5 interaction and subnuclear localization control Arabidopsis root stem cell maintenance",

abstract = "Maintenance and homeostasis of the stem cell niche (SCN) in the Arabidopsis root is essential for growth and development of all root cell types. The SCN is organized around a quiescent center (QC) maintaining the stemness of cells in direct contact. The key transcription factors (TFs) WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and PLETHORAs (PLTs) are expressed in the SCN where they maintain the QC and regulate distal columella stem cell (CSC) fate. Here, we describe the concerted mutual regulation of the key TFs WOX5 and PLTs on a transcriptional and protein interaction level. Additionally, by applying a novel SCN staining method, we demonstrate that both WOX5 and PLTs regulate root SCN homeostasis as they control QC quiescence and CSC fate interdependently. Moreover, we uncover that some PLTs, especially PLT3, contain intrinsically disordered prion-like domains (PrDs) that are necessary for complex formation with WOX5 and its recruitment to subnuclear microdomains/nuclear bodies (NBs) in the CSCs. We propose that this partitioning of PLT-WOX5 complexes to NBs, possibly by phase separation, is important for CSC fate determination.",

author = "Burkart, {Rebecca C} and Strotmann, {Vivien I} and Kirschner, {Gwendolyn Kristin} and Abdullah Akinci and Laura Czempik and Anika Dolata and Alexis Maizel and Stefanie Weidtkamp-Peters and Yvonne Stahl",

note = "KAUST Repository Item: Exported on 2022-04-15 Acknowledgements: We would like to acknowledge funding of R.C.B. by the Deutsche Forschungsgemeinschaft (DFG) through grant Sta1212/1-1 to Y.S. We thank Andrea Bleckmann for sharing the Greengate Cerulean construct, Renze Heidstra, and Ben Scheres for sharing published lines (PLT",

year = "2022",

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doi = "10.15252/embr.202154105",

language = "English (US)",

journal = "EMBO Reports",

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AU - Burkart, Rebecca C

AU - Strotmann, Vivien I

AU - Kirschner, Gwendolyn Kristin

AU - Akinci, Abdullah

AU - Czempik, Laura

AU - Dolata, Anika

AU - Maizel, Alexis

AU - Weidtkamp-Peters, Stefanie

AU - Stahl, Yvonne

N1 - KAUST Repository Item: Exported on 2022-04-15 Acknowledgements: We would like to acknowledge funding of R.C.B. by the Deutsche Forschungsgemeinschaft (DFG) through grant Sta1212/1-1 to Y.S. We thank Andrea Bleckmann for sharing the Greengate Cerulean construct, Renze Heidstra, and Ben Scheres for sharing published lines (PLT

PY - 2022/4/4

Y1 - 2022/4/4

N2 - Maintenance and homeostasis of the stem cell niche (SCN) in the Arabidopsis root is essential for growth and development of all root cell types. The SCN is organized around a quiescent center (QC) maintaining the stemness of cells in direct contact. The key transcription factors (TFs) WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and PLETHORAs (PLTs) are expressed in the SCN where they maintain the QC and regulate distal columella stem cell (CSC) fate. Here, we describe the concerted mutual regulation of the key TFs WOX5 and PLTs on a transcriptional and protein interaction level. Additionally, by applying a novel SCN staining method, we demonstrate that both WOX5 and PLTs regulate root SCN homeostasis as they control QC quiescence and CSC fate interdependently. Moreover, we uncover that some PLTs, especially PLT3, contain intrinsically disordered prion-like domains (PrDs) that are necessary for complex formation with WOX5 and its recruitment to subnuclear microdomains/nuclear bodies (NBs) in the CSCs. We propose that this partitioning of PLT-WOX5 complexes to NBs, possibly by phase separation, is important for CSC fate determination.

AB - Maintenance and homeostasis of the stem cell niche (SCN) in the Arabidopsis root is essential for growth and development of all root cell types. The SCN is organized around a quiescent center (QC) maintaining the stemness of cells in direct contact. The key transcription factors (TFs) WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and PLETHORAs (PLTs) are expressed in the SCN where they maintain the QC and regulate distal columella stem cell (CSC) fate. Here, we describe the concerted mutual regulation of the key TFs WOX5 and PLTs on a transcriptional and protein interaction level. Additionally, by applying a novel SCN staining method, we demonstrate that both WOX5 and PLTs regulate root SCN homeostasis as they control QC quiescence and CSC fate interdependently. Moreover, we uncover that some PLTs, especially PLT3, contain intrinsically disordered prion-like domains (PrDs) that are necessary for complex formation with WOX5 and its recruitment to subnuclear microdomains/nuclear bodies (NBs) in the CSCs. We propose that this partitioning of PLT-WOX5 complexes to NBs, possibly by phase separation, is important for CSC fate determination.

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UR - https://onlinelibrary.wiley.com/doi/10.15252/embr.202154105

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DO - 10.15252/embr.202154105

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SN - 1469-3178

JO - EMBO Reports

JF - EMBO Reports

ER -

PLETHORA-WOX5 interaction and subnuclear localization control Arabidopsis root stem cell maintenance

Abstract

Bibliographical note

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