Phosphorylation of Ser10 of histone H3 regulates chromosome condensation and transcriptional activity. Using time-resolved, high-resolution NMR spectroscopy, we demonstrate that histone H3 Ser10 phosphorylation inhibits checkpoint kinase 1 (Chk1)-and protein kinase C (PKC)-mediated modification of Thr11 and Thr6, the respective primary substrate sites of these kinases. On unmodified H3, both enzymes also target Ser10 and thereby establish autoinhibitory feedback states on individual H3 tails. Whereas phosphorylated Ser10 does not affect acetylation of Lys14 by Gcn5, phosphorylated Thr11 impedes acetylation. Our observations reveal mechanistic hierarchies of H3 phosphorylation and acetylation events and provide a framework for intramolecular modification cross-talk within the N terminus of histone H3.
Bibliographical noteFunding Information:
We thank P. Schmieder and M. Beerbaum for excellent NMR infrastructure maintenance. A.S. and W.F. acknowledge funding by the Max Planck Gesellschaft (MBG). S.J.E. was supported by a Boehringer Ingelheim Fund (BIF) fellowship. F.-X.T. acknowledges support from the Association pour la Recherche contre le Cancer (ARC). D.S. and P.S. are supported by Emmy Noether research grants (SCHW1163/3-1 and SE1794/1-1) from the Deutsche Forschungsgemeinschaft (DFG).
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology