Pheromone receptor of the globally invasive quarantine pest of the palm tree, the red palm weevil ( Rhynchophorus ferrugineus )

Binu Antony, Jibin Johny, Nicolas Montagne, Emmanuelle Jacquin-Joly, Rémi Capoduro, Khasim Cali, Krishna Persaud, Mohammed Ali Al-Saleh, Arnab Pain

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Palm trees are of immense economic, sociocultural, touristic, and patrimonial significance all over the world, and date palm-related knowledge, traditions, and practices are now included in UNESCOs list of the Intangible Cultural Heritage of Humanity. Of all the pests that infest these trees, the red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier), is its primary enemy. The RPW is a category-1 quarantine insect pest that causes enormous economic losses in palm tree cultivation worldwide. The RPW synchronizes mass gathering on the palm tree for feeding and mating, regulated by a male-produced pheromone composed of two methyl-branched compounds, (4RS, 5RS)-4-methylnonan-5-ol (ferrugineol) and 4(RS)-methylnonan-5-one (ferrugineone). Despite the importance of odorant detection in long-range orientation towards palm trees, palm colonization, and mating, the pheromone receptor has not been identified in this species. In this study, we report the identification and characterization of the first RPW pheromone receptor, RferOR1. Using gene silencing and functional expression in Drosophila olfactory receptor neurons, we demonstrate that RferOR1 is tuned to ferrugineol and ferrugineone and binds five other structurally related molecules. We reveal the lifetime expression of RferOR1, which correlates with adult mating success irrespective of age, a factor that could explain the wide distribution and spread of this pest. As palm weevils are challenging to control based on conventional methods, elucidation of the mechanisms of pheromone detection opens new routes for mating disruption and the early detection of this pest via the development of pheromone receptor-based biosensors.
Original languageEnglish (US)
JournalMolecular Ecology
DOIs
StatePublished - Mar 9 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-03-30
Acknowledgements: Funding for this research—grant numbers: KACST-NSTIP 12-AGR2854-02 (MAARIFAH-KACST), KAUST-OSR-2018-RPW-3816–1, and OSR-2018-RPW-3816–4 of Saudi Arabia, and the ANR Investissements d'avenir program "PheroSensor" of France. The authors are grateful to the Deanship of Scientific Research, King Saud University, for funding through the Vice Deanship of Scientific Research Chairs. The authors thank Anne-Francoise J. Lamblin and T. A. Abrajano of KAUST-OSR for their invaluable support. The authors thank the date palm farmers in the Al Kharj and Al Qassim areas for their support in obtaining RPWs and advice in adult weevil collection. Jibin Johny is grateful for the researcher stipend and training provided by CDPR through 12-AGR2854-02 and OSR-2018-RPW-3816–1 projects. We also thank Jérémy Gévar (iEES, France) for GC-FID/MS analyses of standard compounds.

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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