Peroxidase-like activity of Fe3O4 nanoparticles and Fe3O4-graphene oxide nanohybrids: Effect of the amino- and carboxyl-surface modifications on H2O2 sensing

Panagiotis Bilalis; Evdokia Karagouni; Dimitra K. Toubanaki

doi:10.1002/aoc.6803

Peroxidase-like activity of Fe3O4 nanoparticles and Fe3O4-graphene oxide nanohybrids: Effect of the amino- and carboxyl-surface modifications on H2O2 sensing

Panagiotis Bilalis, Evdokia Karagouni, Dimitra K. Toubanaki

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Magnetic, iron oxide nanoparticles and graphene-iron oxide nanohybrids have been introduced as artificial peroxidase-mimics. In the present paper, the impact of coating with different functional groups on the peroxidase activity of both nanoparticles and nanohybrids, was studied. Magnetite nanoparticles and graphene-iron oxide nanohybrids, with -amino or -carboxyl terminal groups were synthesized and fully characterized by FTIR, TGA, TEM, XRD, VSM and zeta potential. All particle formulations were evaluated for peroxidase activity, by studying the oxidation reaction of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2). It was found that all tested formulations retained their catalytic ability (>70%) at 95 °C, at wide pH range, and were reusable with 60% of their catalytic activity after 10 uses. All nanomaterials were found to follow Michaelis–Menten kinetics and peroxidase activity varied with respect to electrostatic affinity between nanoparticles or nanohybrids and the peroxidase substrates, evidenced by differences in determined kinetic parameters. The Km values of all tested particles with H2O2 substrate, were higher than those reported for HRP (0.214-6.36 mM), while the calculated values of reaction rates (Vmax) for the studied nanoparticles and nanohybrids with TMB and H2O2 as substrates were comparable with those reported for HRP. Our findings confirm that iron oxide nanoparticles and graphene based metallic nanohybrids can be tailor-made to possess improved peroxidase-like activity. Such enhancements could further widen nanohybrids application in a wide range of biomedical applications, including H2O2 detection in a proof-of-concept assay, which confirmed low detection limits of the tested formulations (5.3 – 11.3 μM).

Original language	English (US)
Journal	Applied Organometallic Chemistry
DOIs	https://doi.org/10.1002/aoc.6803
State	Published - Jun 29 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-07-01
Acknowledgements: This research was partially funded within the framework of the Action “Supporting Postdoctoral Researchers”(grant number LS9_488) of the Operational Program "Education and Lifelong Learning" (Action’s Beneficiary: General Secretariat for Research and Technology), and was co-financed by the European Social Fund (ESF) and the Greek State. A. Sklavounos and Prof. A.C. Calokerinos (National and Kapodistrian University of Athens, Department of Chemistry) are acknowledged for assisting with the ultraviolet–visible spectroscopy measurements. Dr M. Agallou (Hellenic Pasteur Institute) is greatly acknowledged for helpful discussions on data analysis.

ASJC Scopus subject areas

General Chemistry
Inorganic Chemistry

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https://repository.kaust.edu.sa/bitstream/10754/679531/1/Applied%20Organom%20Chemis%20-%202022%20-%20Bilalis%20-%20Peroxidase%e2%80%90like%20activity%20of%20Fe3O4%20nanoparticles%20and%20Fe3O4%e2%80%90graphene%20oxide.pdf

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@article{23bb0d7affd8451cbdbb1f32d4157d29,

title = "Peroxidase-like activity of Fe3O4 nanoparticles and Fe3O4-graphene oxide nanohybrids: Effect of the amino- and carboxyl-surface modifications on H2O2 sensing",

abstract = "Magnetic, iron oxide nanoparticles and graphene-iron oxide nanohybrids have been introduced as artificial peroxidase-mimics. In the present paper, the impact of coating with different functional groups on the peroxidase activity of both nanoparticles and nanohybrids, was studied. Magnetite nanoparticles and graphene-iron oxide nanohybrids, with -amino or -carboxyl terminal groups were synthesized and fully characterized by FTIR, TGA, TEM, XRD, VSM and zeta potential. All particle formulations were evaluated for peroxidase activity, by studying the oxidation reaction of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2). It was found that all tested formulations retained their catalytic ability (>70%) at 95 °C, at wide pH range, and were reusable with 60% of their catalytic activity after 10 uses. All nanomaterials were found to follow Michaelis–Menten kinetics and peroxidase activity varied with respect to electrostatic affinity between nanoparticles or nanohybrids and the peroxidase substrates, evidenced by differences in determined kinetic parameters. The Km values of all tested particles with H2O2 substrate, were higher than those reported for HRP (0.214-6.36 mM), while the calculated values of reaction rates (Vmax) for the studied nanoparticles and nanohybrids with TMB and H2O2 as substrates were comparable with those reported for HRP. Our findings confirm that iron oxide nanoparticles and graphene based metallic nanohybrids can be tailor-made to possess improved peroxidase-like activity. Such enhancements could further widen nanohybrids application in a wide range of biomedical applications, including H2O2 detection in a proof-of-concept assay, which confirmed low detection limits of the tested formulations (5.3 – 11.3 μM).",

author = "Panagiotis Bilalis and Evdokia Karagouni and Toubanaki, {Dimitra K.}",

note = "KAUST Repository Item: Exported on 2022-07-01 Acknowledgements: This research was partially funded within the framework of the Action “Supporting Postdoctoral Researchers”(grant number LS9_488) of the Operational Program {"}Education and Lifelong Learning{"} (Action{\textquoteright}s Beneficiary: General Secretariat for Research and Technology), and was co-financed by the European Social Fund (ESF) and the Greek State. A. Sklavounos and Prof. A.C. Calokerinos (National and Kapodistrian University of Athens, Department of Chemistry) are acknowledged for assisting with the ultraviolet–visible spectroscopy measurements. Dr M. Agallou (Hellenic Pasteur Institute) is greatly acknowledged for helpful discussions on data analysis.",

year = "2022",

month = jun,

day = "29",

doi = "10.1002/aoc.6803",

language = "English (US)",

journal = "Applied Organometallic Chemistry",

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T1 - Peroxidase-like activity of Fe3O4 nanoparticles and Fe3O4-graphene oxide nanohybrids: Effect of the amino- and carboxyl-surface modifications on H2O2 sensing

AU - Bilalis, Panagiotis

AU - Karagouni, Evdokia

AU - Toubanaki, Dimitra K.

N1 - KAUST Repository Item: Exported on 2022-07-01 Acknowledgements: This research was partially funded within the framework of the Action “Supporting Postdoctoral Researchers”(grant number LS9_488) of the Operational Program "Education and Lifelong Learning" (Action’s Beneficiary: General Secretariat for Research and Technology), and was co-financed by the European Social Fund (ESF) and the Greek State. A. Sklavounos and Prof. A.C. Calokerinos (National and Kapodistrian University of Athens, Department of Chemistry) are acknowledged for assisting with the ultraviolet–visible spectroscopy measurements. Dr M. Agallou (Hellenic Pasteur Institute) is greatly acknowledged for helpful discussions on data analysis.

PY - 2022/6/29

Y1 - 2022/6/29

N2 - Magnetic, iron oxide nanoparticles and graphene-iron oxide nanohybrids have been introduced as artificial peroxidase-mimics. In the present paper, the impact of coating with different functional groups on the peroxidase activity of both nanoparticles and nanohybrids, was studied. Magnetite nanoparticles and graphene-iron oxide nanohybrids, with -amino or -carboxyl terminal groups were synthesized and fully characterized by FTIR, TGA, TEM, XRD, VSM and zeta potential. All particle formulations were evaluated for peroxidase activity, by studying the oxidation reaction of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2). It was found that all tested formulations retained their catalytic ability (>70%) at 95 °C, at wide pH range, and were reusable with 60% of their catalytic activity after 10 uses. All nanomaterials were found to follow Michaelis–Menten kinetics and peroxidase activity varied with respect to electrostatic affinity between nanoparticles or nanohybrids and the peroxidase substrates, evidenced by differences in determined kinetic parameters. The Km values of all tested particles with H2O2 substrate, were higher than those reported for HRP (0.214-6.36 mM), while the calculated values of reaction rates (Vmax) for the studied nanoparticles and nanohybrids with TMB and H2O2 as substrates were comparable with those reported for HRP. Our findings confirm that iron oxide nanoparticles and graphene based metallic nanohybrids can be tailor-made to possess improved peroxidase-like activity. Such enhancements could further widen nanohybrids application in a wide range of biomedical applications, including H2O2 detection in a proof-of-concept assay, which confirmed low detection limits of the tested formulations (5.3 – 11.3 μM).

AB - Magnetic, iron oxide nanoparticles and graphene-iron oxide nanohybrids have been introduced as artificial peroxidase-mimics. In the present paper, the impact of coating with different functional groups on the peroxidase activity of both nanoparticles and nanohybrids, was studied. Magnetite nanoparticles and graphene-iron oxide nanohybrids, with -amino or -carboxyl terminal groups were synthesized and fully characterized by FTIR, TGA, TEM, XRD, VSM and zeta potential. All particle formulations were evaluated for peroxidase activity, by studying the oxidation reaction of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2). It was found that all tested formulations retained their catalytic ability (>70%) at 95 °C, at wide pH range, and were reusable with 60% of their catalytic activity after 10 uses. All nanomaterials were found to follow Michaelis–Menten kinetics and peroxidase activity varied with respect to electrostatic affinity between nanoparticles or nanohybrids and the peroxidase substrates, evidenced by differences in determined kinetic parameters. The Km values of all tested particles with H2O2 substrate, were higher than those reported for HRP (0.214-6.36 mM), while the calculated values of reaction rates (Vmax) for the studied nanoparticles and nanohybrids with TMB and H2O2 as substrates were comparable with those reported for HRP. Our findings confirm that iron oxide nanoparticles and graphene based metallic nanohybrids can be tailor-made to possess improved peroxidase-like activity. Such enhancements could further widen nanohybrids application in a wide range of biomedical applications, including H2O2 detection in a proof-of-concept assay, which confirmed low detection limits of the tested formulations (5.3 – 11.3 μM).

UR - http://hdl.handle.net/10754/679531

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DO - 10.1002/aoc.6803

M3 - Article

SN - 0268-2605

JO - Applied Organometallic Chemistry

JF - Applied Organometallic Chemistry

ER -

Peroxidase-like activity of Fe3O4 nanoparticles and Fe3O4-graphene oxide nanohybrids: Effect of the amino- and carboxyl-surface modifications on H2O2 sensing

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