Performance characteristics of a novel tandem mass spectrometry assay for serum testosterone

Mark M. Kushnir; Alan L. Rockwood; William L. Roberts; Elizabeth G. Pattison; Ashley M. Bunker; Robert L. Fitzgerald; A. Wayne Meikle

doi:10.1373/clinchem.2005.052167

Performance characteristics of a novel tandem mass spectrometry assay for serum testosterone

Mark M. Kushnir, Alan L. Rockwood, William L. Roberts, Elizabeth G. Pattison, Ashley M. Bunker, Robert L. Fitzgerald, A. Wayne Meikle

Research output: Contribution to journal › Article › peer-review

151 Scopus citations

Abstract

Background: Commercial immunoassays for testosterone (Te) may give inaccurate results for samples from women and children, leading to misdiagosis and inappropriate treatment. We developed a sensitive and specific tandem mass spectrometric assay for measurement of Te at the concentrations encountered in women and children. Methods: Te was extracted with methyl tert-butyl ether from 100 μL of serum or plasma, derivatized to form an oxime, and reextracted by solid-phase extraction. Instrumental analysis was performed on an API 4000 HPLC tandem mass spectrometer in the multiple-reaction monitoring (MRM) mode. The MRM transitions (m/z) were 304→124 and 304→112 for Te and 307→124 and 307→112 for d3-Te. Results: Within- and between-run CVs were

Original language	English (US)
Pages (from-to)	120-128
Number of pages	9
Journal	Clinical Chemistry
Volume	52
Issue number	1
DOIs	https://doi.org/10.1373/clinchem.2005.052167
State	Published - Jan 1 2006
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-09-20

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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10.1373/clinchem.2005.052167

Cite this

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title = "Performance characteristics of a novel tandem mass spectrometry assay for serum testosterone",

abstract = "Background: Commercial immunoassays for testosterone (Te) may give inaccurate results for samples from women and children, leading to misdiagosis and inappropriate treatment. We developed a sensitive and specific tandem mass spectrometric assay for measurement of Te at the concentrations encountered in women and children. Methods: Te was extracted with methyl tert-butyl ether from 100 μL of serum or plasma, derivatized to form an oxime, and reextracted by solid-phase extraction. Instrumental analysis was performed on an API 4000 HPLC tandem mass spectrometer in the multiple-reaction monitoring (MRM) mode. The MRM transitions (m/z) were 304→124 and 304→112 for Te and 307→124 and 307→112 for d3-Te. Results: Within- and between-run CVs were",

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doi = "10.1373/clinchem.2005.052167",

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AU - Kushnir, Mark M.

AU - Rockwood, Alan L.

AU - Roberts, William L.

AU - Pattison, Elizabeth G.

AU - Bunker, Ashley M.

AU - Fitzgerald, Robert L.

AU - Meikle, A. Wayne

N1 - Generated from Scopus record by KAUST IRTS on 2023-09-20

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Background: Commercial immunoassays for testosterone (Te) may give inaccurate results for samples from women and children, leading to misdiagosis and inappropriate treatment. We developed a sensitive and specific tandem mass spectrometric assay for measurement of Te at the concentrations encountered in women and children. Methods: Te was extracted with methyl tert-butyl ether from 100 μL of serum or plasma, derivatized to form an oxime, and reextracted by solid-phase extraction. Instrumental analysis was performed on an API 4000 HPLC tandem mass spectrometer in the multiple-reaction monitoring (MRM) mode. The MRM transitions (m/z) were 304→124 and 304→112 for Te and 307→124 and 307→112 for d3-Te. Results: Within- and between-run CVs were

AB - Background: Commercial immunoassays for testosterone (Te) may give inaccurate results for samples from women and children, leading to misdiagosis and inappropriate treatment. We developed a sensitive and specific tandem mass spectrometric assay for measurement of Te at the concentrations encountered in women and children. Methods: Te was extracted with methyl tert-butyl ether from 100 μL of serum or plasma, derivatized to form an oxime, and reextracted by solid-phase extraction. Instrumental analysis was performed on an API 4000 HPLC tandem mass spectrometer in the multiple-reaction monitoring (MRM) mode. The MRM transitions (m/z) were 304→124 and 304→112 for Te and 307→124 and 307→112 for d3-Te. Results: Within- and between-run CVs were

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Performance characteristics of a novel tandem mass spectrometry assay for serum testosterone

Abstract

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