Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models

Wafaa Arab; Hepi Hari Susapto; Dana Majed Alhattab; Charlotte Hauser

doi:10.1177/20417314221111868

Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models

Wafaa Arab, Hepi Hari Susapto, Dana Majed Alhattab, Charlotte Hauser

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Millions of people worldwide suffer from skin injuries, which create significant problems in their lives and are costly to cure. Tissue engineering is a promising approach that aims to fabricate functional organs using biocompatible scaffolds. We designed ultrashort tetrameric peptides with promising properties required for skin tissue engineering. Our work aimed to test the efficacy of these scaffolds for the fabrication of dermal grafts and 3D vascularized skin tissue models. We found that the direct contact of keratinocytes and fibroblasts enhanced the proliferation of the keratinocytes. Moreover, the expression levels of TGF-β1, b-FGF, IL-6, and IL-1α is correlated with the growth of the fibroblasts and keratinocytes in the co-culture. Furthermore, we successfully produced a 3D vascularized skin co-culture model using these peptide scaffolds. We believe that the described results represent an advancement in the fabrication of skin tissue equivalent, thereby providing the opportunity to rebuild missing, failing, or damaged parts.

Original language	English (US)
Pages (from-to)	204173142211118
Journal	Journal of Tissue Engineering
Volume	13
DOIs	https://doi.org/10.1177/20417314221111868
State	Published - Jul 29 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-09-14
Acknowledgements: The authors would like to acknowledge the support from Ohoud Alharbi, KAUST Core Laboratories, for SEM imaging. We also acknowledge Manola Moretti from King Abdullah University of Science and Technology for the valuable discussions and suggestions. Finally, we also acknowledge Prof. Abdalla Awidi from the University of Jordan/Cell Therapy Center for providing primary human keratinocytes. This work was financially supported by King Abdullah University of Science and Technology (KAUST).

ASJC Scopus subject areas

Biomaterials
Biomedical Engineering
Medicine (miscellaneous)

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10.1177/20417314221111868

https://repository.kaust.edu.sa/bitstream/10754/679959/1/20417314221111868.pdf

Cite this

@article{b3e3d69dc5084cd59cfb92442a22180c,

title = "Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models",

abstract = "Millions of people worldwide suffer from skin injuries, which create significant problems in their lives and are costly to cure. Tissue engineering is a promising approach that aims to fabricate functional organs using biocompatible scaffolds. We designed ultrashort tetrameric peptides with promising properties required for skin tissue engineering. Our work aimed to test the efficacy of these scaffolds for the fabrication of dermal grafts and 3D vascularized skin tissue models. We found that the direct contact of keratinocytes and fibroblasts enhanced the proliferation of the keratinocytes. Moreover, the expression levels of TGF-β1, b-FGF, IL-6, and IL-1α is correlated with the growth of the fibroblasts and keratinocytes in the co-culture. Furthermore, we successfully produced a 3D vascularized skin co-culture model using these peptide scaffolds. We believe that the described results represent an advancement in the fabrication of skin tissue equivalent, thereby providing the opportunity to rebuild missing, failing, or damaged parts.",

author = "Wafaa Arab and Susapto, {Hepi Hari} and Alhattab, {Dana Majed} and Charlotte Hauser",

note = "KAUST Repository Item: Exported on 2022-09-14 Acknowledgements: The authors would like to acknowledge the support from Ohoud Alharbi, KAUST Core Laboratories, for SEM imaging. We also acknowledge Manola Moretti from King Abdullah University of Science and Technology for the valuable discussions and suggestions. Finally, we also acknowledge Prof. Abdalla Awidi from the University of Jordan/Cell Therapy Center for providing primary human keratinocytes. This work was financially supported by King Abdullah University of Science and Technology (KAUST).",

year = "2022",

month = jul,

day = "29",

doi = "10.1177/20417314221111868",

language = "English (US)",

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journal = "Journal of Tissue Engineering",

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T1 - Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models

AU - Arab, Wafaa

AU - Susapto, Hepi Hari

AU - Alhattab, Dana Majed

AU - Hauser, Charlotte

N1 - KAUST Repository Item: Exported on 2022-09-14 Acknowledgements: The authors would like to acknowledge the support from Ohoud Alharbi, KAUST Core Laboratories, for SEM imaging. We also acknowledge Manola Moretti from King Abdullah University of Science and Technology for the valuable discussions and suggestions. Finally, we also acknowledge Prof. Abdalla Awidi from the University of Jordan/Cell Therapy Center for providing primary human keratinocytes. This work was financially supported by King Abdullah University of Science and Technology (KAUST).

PY - 2022/7/29

Y1 - 2022/7/29

N2 - Millions of people worldwide suffer from skin injuries, which create significant problems in their lives and are costly to cure. Tissue engineering is a promising approach that aims to fabricate functional organs using biocompatible scaffolds. We designed ultrashort tetrameric peptides with promising properties required for skin tissue engineering. Our work aimed to test the efficacy of these scaffolds for the fabrication of dermal grafts and 3D vascularized skin tissue models. We found that the direct contact of keratinocytes and fibroblasts enhanced the proliferation of the keratinocytes. Moreover, the expression levels of TGF-β1, b-FGF, IL-6, and IL-1α is correlated with the growth of the fibroblasts and keratinocytes in the co-culture. Furthermore, we successfully produced a 3D vascularized skin co-culture model using these peptide scaffolds. We believe that the described results represent an advancement in the fabrication of skin tissue equivalent, thereby providing the opportunity to rebuild missing, failing, or damaged parts.

AB - Millions of people worldwide suffer from skin injuries, which create significant problems in their lives and are costly to cure. Tissue engineering is a promising approach that aims to fabricate functional organs using biocompatible scaffolds. We designed ultrashort tetrameric peptides with promising properties required for skin tissue engineering. Our work aimed to test the efficacy of these scaffolds for the fabrication of dermal grafts and 3D vascularized skin tissue models. We found that the direct contact of keratinocytes and fibroblasts enhanced the proliferation of the keratinocytes. Moreover, the expression levels of TGF-β1, b-FGF, IL-6, and IL-1α is correlated with the growth of the fibroblasts and keratinocytes in the co-culture. Furthermore, we successfully produced a 3D vascularized skin co-culture model using these peptide scaffolds. We believe that the described results represent an advancement in the fabrication of skin tissue equivalent, thereby providing the opportunity to rebuild missing, failing, or damaged parts.

UR - http://hdl.handle.net/10754/679959

UR - http://journals.sagepub.com/doi/10.1177/20417314221111868

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DO - 10.1177/20417314221111868

M3 - Article

C2 - 35923174

SN - 2041-7314

VL - 13

SP - 204173142211118

JO - Journal of Tissue Engineering

JF - Journal of Tissue Engineering

ER -

Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models

Abstract

Bibliographical note

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