Optical tweezers for sample fixing in micro-diffraction experiments

H. Amenitsch; D. Cojoc; M. Rappolt; B. Sartori; P. Laggner; E. Ferrari; V. Garbin; M. Burghammer; Ch Riekel; E. Di Fabrizio

doi:10.1063/1.2436299

Optical tweezers for sample fixing in micro-diffraction experiments

H. Amenitsch^*, D. Cojoc, M. Rappolt, B. Sartori, P. Laggner, E. Ferrari, V. Garbin, M. Burghammer, Ch Riekel, E. Di Fabrizio

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.

Original language	English (US)
Title of host publication	SYNCHROTRON RADIATION INSTRUMENTATION
Subtitle of host publication	Ninth International Conference on Synchrotron Radiation Instrumentation
Pages	1287-1290
Number of pages	4
Volume	879
DOIs	https://doi.org/10.1063/1.2436299
State	Published - 2007
Externally published	Yes
Event	SYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation - Daegu, Korea, Republic of Duration: May 28 2006 → Jun 28 2006

Other

Other	SYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation
Country/Territory	Korea, Republic of
City	Daegu
Period	05/28/06 → 06/28/06

Keywords

Optical manipulation
Phospholipids
Single biological entity
X-ray micro diffraction

ASJC Scopus subject areas

Physics and Astronomy(all)

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10.1063/1.2436299

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Amenitsch, H., Cojoc, D., Rappolt, M., Sartori, B., Laggner, P., Ferrari, E., Garbin, V., Burghammer, M., Riekel, C., & Di Fabrizio, E. (2007). Optical tweezers for sample fixing in micro-diffraction experiments. In SYNCHROTRON RADIATION INSTRUMENTATION: Ninth International Conference on Synchrotron Radiation Instrumentation (Vol. 879, pp. 1287-1290) https://doi.org/10.1063/1.2436299

@inproceedings{395c5ad1012840a68e8e353ad6bd550e,

title = "Optical tweezers for sample fixing in micro-diffraction experiments",

abstract = "In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.",

keywords = "Optical manipulation, Phospholipids, Single biological entity, X-ray micro diffraction",

author = "H. Amenitsch and D. Cojoc and M. Rappolt and B. Sartori and P. Laggner and E. Ferrari and V. Garbin and M. Burghammer and Ch Riekel and {Di Fabrizio}, E.",

year = "2007",

doi = "10.1063/1.2436299",

language = "English (US)",

isbn = "0735403732",

volume = "879",

pages = "1287--1290",

booktitle = "SYNCHROTRON RADIATION INSTRUMENTATION",

note = "SYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation ; Conference date: 28-05-2006 Through 28-06-2006",

}

Amenitsch, H, Cojoc, D, Rappolt, M, Sartori, B, Laggner, P, Ferrari, E, Garbin, V, Burghammer, M, Riekel, C & Di Fabrizio, E 2007, Optical tweezers for sample fixing in micro-diffraction experiments. in SYNCHROTRON RADIATION INSTRUMENTATION: Ninth International Conference on Synchrotron Radiation Instrumentation. vol. 879, pp. 1287-1290, SYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation, Daegu, Korea, Republic of, 05/28/06. https://doi.org/10.1063/1.2436299

TY - GEN

T1 - Optical tweezers for sample fixing in micro-diffraction experiments

AU - Amenitsch, H.

AU - Cojoc, D.

AU - Rappolt, M.

AU - Sartori, B.

AU - Laggner, P.

AU - Ferrari, E.

AU - Garbin, V.

AU - Burghammer, M.

AU - Riekel, Ch

AU - Di Fabrizio, E.

PY - 2007

Y1 - 2007

N2 - In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.

AB - In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.

KW - Optical manipulation

KW - Phospholipids

KW - Single biological entity

KW - X-ray micro diffraction

UR - http://www.scopus.com/inward/record.url?scp=33947379515&partnerID=8YFLogxK

U2 - 10.1063/1.2436299

DO - 10.1063/1.2436299

M3 - Conference contribution

AN - SCOPUS:33947379515

SN - 0735403732

SN - 9780735403734

VL - 879

SP - 1287

EP - 1290

BT - SYNCHROTRON RADIATION INSTRUMENTATION

T2 - SYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation

Y2 - 28 May 2006 through 28 June 2006

ER -

Optical tweezers for sample fixing in micro-diffraction experiments

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