@inproceedings{82bcd661f99b404f8bcc5639fb984217,
title = "On-chip study of the drug response of MCF-7 breast cancer tumour spheroids under chronic and cycling hypoxia",
abstract = "We present a novel study of the response of MCF-7 tumour spheroids to the anticancer agent doxorubicin under normoxic as well as chronic and transient hypoxic conditions. Viability is monitored after a 72h treatment under 20% (normoxia), 1% (hypoxia), and 0.1% (anoxia) chronic O2 as well as under a cycling profile of 4h at 0% and 4h at 10% O2. We use a microfluidic oxygen control device to expose spheroids to varying O2 conditions and analyze their response with brightfield, widefield fluorescence, and two-photon microscopy (TPM), observing doxorubicin resistance in hypoxic spheroids.",
keywords = "3-D cell culture, Drug screening, Microfluidics, Transient/cycling hypoxia",
author = "Grist, {S. M.} and Nasseri, {S. S.} and L. Laplatine and A. D{\'i}az-Gaxiola and Liu, {M. C.} and L. Chrostowski and Cheung, {K. C.}",
year = "2016",
language = "English (US)",
series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
publisher = "Chemical and Biological Microsystems Society",
pages = "403--404",
booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",
}
