On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization

Chinthaka Pasan Gooneratne; Rimantas Kodzius; Fuquan Li; Ian G. Foulds; Jürgen Kosel

doi:10.3390/s16091369

On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization

Chinthaka Pasan Gooneratne, Rimantas Kodzius, Fuquan Li, Ian G. Foulds, Jürgen Kosel

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The remarkable advantages micro-chip platforms offer over cumbersome, time-consuming equipment currently in use for bio-analysis are well documented. In this research, a micro-chip that includes a unique magnetic actuator (MA) for the manipulation of superparamagnetic beads (SPBs), and a magnetoresistive sensor for the detection of SPBs is presented. A design methodology, which takes into account the magnetic volume of SPBs, diffusion and heat transfer phenomena, is presented with the aid of numerical analysis to optimize the parameters of the MA. The MA was employed as a magnetic flux generator and experimental analysis with commercially available COMPEL™ and Dynabeads® demonstrated the ability of the MA to precisely transport a small number of SPBs over long distances and concentrate SPBs to a sensing site for detection. Moreover, the velocities of COMPEL™ and Dynabead® SPBs were correlated to their magnetic volumes and were in good agreement with numerical model predictions. We found that 2.8 μm Dynabeads® travel faster, and can be attracted to a magnetic source from a longer distance, than 6.2 μm COMPEL™ beads at magnetic flux magnitudes of less than 10 mT. The micro-chip system could easily be integrated with electronic circuitry and microfluidic functions, paving the way for an on-chip biomolecule quantification device

Original language	English (US)
Pages (from-to)	1369
Journal	Sensors
Volume	16
Issue number	9
DOIs	https://doi.org/10.3390/s16091369
State	Published - Aug 26 2016

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: Research reported in this publication was supported by King Abdullah University of Science and Technology (KAUST). The authors acknowledge Filipe Cardoso of INESC Microsistemas & Nanotecnologias (INESC MN) for his help with the TMR sensor fabrication. The authors would like to thank Ren Jian, Zhihong Wang, Basil Chew and Xiang Yu, for their help with microfabrication and material characterization. The authors would also like to thank Professor Nicole Pamme, Professor Quentin Pankhurst, Professor Martin Gijs, Professor Menno Prins, Mark Tarn and Chengxun Liu for fruitful discussions on the research

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10.3390/s16091369

https://repository.kaust.edu.sa/bitstream/10754/621879/1/sensors-16-01369.pdf

Cite this

@article{d1fe330f2c6a45a1a2934a59fdca56f1,

title = "On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization",

abstract = "The remarkable advantages micro-chip platforms offer over cumbersome, time-consuming equipment currently in use for bio-analysis are well documented. In this research, a micro-chip that includes a unique magnetic actuator (MA) for the manipulation of superparamagnetic beads (SPBs), and a magnetoresistive sensor for the detection of SPBs is presented. A design methodology, which takes into account the magnetic volume of SPBs, diffusion and heat transfer phenomena, is presented with the aid of numerical analysis to optimize the parameters of the MA. The MA was employed as a magnetic flux generator and experimental analysis with commercially available COMPEL{\texttrademark} and Dynabeads{\textregistered} demonstrated the ability of the MA to precisely transport a small number of SPBs over long distances and concentrate SPBs to a sensing site for detection. Moreover, the velocities of COMPEL{\texttrademark} and Dynabead{\textregistered} SPBs were correlated to their magnetic volumes and were in good agreement with numerical model predictions. We found that 2.8 μm Dynabeads{\textregistered} travel faster, and can be attracted to a magnetic source from a longer distance, than 6.2 μm COMPEL{\texttrademark} beads at magnetic flux magnitudes of less than 10 mT. The micro-chip system could easily be integrated with electronic circuitry and microfluidic functions, paving the way for an on-chip biomolecule quantification device",

author = "Gooneratne, {Chinthaka Pasan} and Rimantas Kodzius and Fuquan Li and Foulds, {Ian G.} and J{\"u}rgen Kosel",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Research reported in this publication was supported by King Abdullah University of Science and Technology (KAUST). The authors acknowledge Filipe Cardoso of INESC Microsistemas & Nanotecnologias (INESC MN) for his help with the TMR sensor fabrication. The authors would like to thank Ren Jian, Zhihong Wang, Basil Chew and Xiang Yu, for their help with microfabrication and material characterization. The authors would also like to thank Professor Nicole Pamme, Professor Quentin Pankhurst, Professor Martin Gijs, Professor Menno Prins, Mark Tarn and Chengxun Liu for fruitful discussions on the research",

year = "2016",

month = aug,

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doi = "10.3390/s16091369",

language = "English (US)",

volume = "16",

pages = "1369",

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T1 - On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization

AU - Gooneratne, Chinthaka Pasan

AU - Kodzius, Rimantas

AU - Li, Fuquan

AU - Foulds, Ian G.

AU - Kosel, Jürgen

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Research reported in this publication was supported by King Abdullah University of Science and Technology (KAUST). The authors acknowledge Filipe Cardoso of INESC Microsistemas & Nanotecnologias (INESC MN) for his help with the TMR sensor fabrication. The authors would like to thank Ren Jian, Zhihong Wang, Basil Chew and Xiang Yu, for their help with microfabrication and material characterization. The authors would also like to thank Professor Nicole Pamme, Professor Quentin Pankhurst, Professor Martin Gijs, Professor Menno Prins, Mark Tarn and Chengxun Liu for fruitful discussions on the research

PY - 2016/8/26

Y1 - 2016/8/26

N2 - The remarkable advantages micro-chip platforms offer over cumbersome, time-consuming equipment currently in use for bio-analysis are well documented. In this research, a micro-chip that includes a unique magnetic actuator (MA) for the manipulation of superparamagnetic beads (SPBs), and a magnetoresistive sensor for the detection of SPBs is presented. A design methodology, which takes into account the magnetic volume of SPBs, diffusion and heat transfer phenomena, is presented with the aid of numerical analysis to optimize the parameters of the MA. The MA was employed as a magnetic flux generator and experimental analysis with commercially available COMPEL™ and Dynabeads® demonstrated the ability of the MA to precisely transport a small number of SPBs over long distances and concentrate SPBs to a sensing site for detection. Moreover, the velocities of COMPEL™ and Dynabead® SPBs were correlated to their magnetic volumes and were in good agreement with numerical model predictions. We found that 2.8 μm Dynabeads® travel faster, and can be attracted to a magnetic source from a longer distance, than 6.2 μm COMPEL™ beads at magnetic flux magnitudes of less than 10 mT. The micro-chip system could easily be integrated with electronic circuitry and microfluidic functions, paving the way for an on-chip biomolecule quantification device

AB - The remarkable advantages micro-chip platforms offer over cumbersome, time-consuming equipment currently in use for bio-analysis are well documented. In this research, a micro-chip that includes a unique magnetic actuator (MA) for the manipulation of superparamagnetic beads (SPBs), and a magnetoresistive sensor for the detection of SPBs is presented. A design methodology, which takes into account the magnetic volume of SPBs, diffusion and heat transfer phenomena, is presented with the aid of numerical analysis to optimize the parameters of the MA. The MA was employed as a magnetic flux generator and experimental analysis with commercially available COMPEL™ and Dynabeads® demonstrated the ability of the MA to precisely transport a small number of SPBs over long distances and concentrate SPBs to a sensing site for detection. Moreover, the velocities of COMPEL™ and Dynabead® SPBs were correlated to their magnetic volumes and were in good agreement with numerical model predictions. We found that 2.8 μm Dynabeads® travel faster, and can be attracted to a magnetic source from a longer distance, than 6.2 μm COMPEL™ beads at magnetic flux magnitudes of less than 10 mT. The micro-chip system could easily be integrated with electronic circuitry and microfluidic functions, paving the way for an on-chip biomolecule quantification device

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UR - http://www.mdpi.com/1424-8220/16/9/1369

UR - http://www.scopus.com/inward/record.url?scp=84984845741&partnerID=8YFLogxK

U2 - 10.3390/s16091369

DO - 10.3390/s16091369

M3 - Article

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SN - 1424-8220

VL - 16

SP - 1369

JO - Sensors

JF - Sensors

IS - 9

ER -

On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization

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