Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast

Muhammad Tehseen, Chunyan Liao, Helen Dacres, Mira Dumancic, Stephen Trowell, Alisha Anderson

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.
Original languageEnglish (US)
Pages (from-to)e108680
JournalPLoS ONE
Issue number9
StatePublished - Sep 25 2014

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The research is funded by CSIRO. However, the funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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